ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2013
Multiplexed analysis of steroid hormones in human serum using novel microflow tile technology and LC-MS/MS.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Gonadotropin-releasing hormone agonist in premenopausal women does not alter hypothalamic-pituitary-adrenal axis response to corticotropin-releasing hormone.American journal of physiology. Endocrinology and metabolism · 2018Trial
- The Influence of Remnant Cholesterol on Cardiovascular Risk and Mortality in Patients with Non-Functional Adrenal Incidentalomas and Mild Autonomous Cortisol Secretion: A Retrospective Cohort Study.Journal of clinical medicine · 2024Article
- Polypyrrole as Adsorbent in Magnetic Solid Phase Extraction for Progesterone Determination from Human Plasma.ACS omega · 2024Article
- Article
- Plasma, urine, and stool metabolites in response to dietary rice bran and navy bean supplementation in adults at high-risk for colorectal cancer.Frontiers in gastroenterology (Lausanne, Switzerland) · 2023Article
- Rapidity and Precision of Steroid Hormone Measurement.Journal of clinical medicine · 2022Review
- The impact of extraction protocol on the chemical profile of cannabis extracts from a single cultivar.Scientific reports · 2021Article
- Daily Rice Bran Consumption for 6 Months Influences Serum Glucagon-Like Peptide 2 and Metabolite Profiles without Differences in Trace Elements and Heavy Metals in Weaning Nicaraguan Infants at 12 Months of Age.Current developments in nutrition · 2021Article
- Comprehensive Evaluation of Metabolites and Minerals in 6 Microgreen Species and the Influence of Maturity.Current developments in nutrition · 2021Article
- Longitudinal Multi-omics Reveals Subset-Specific Mechanisms Underlying Irritable Bowel Syndrome.Cell · 2020Article
- Non-invasive Drug Monitoring of β-Lactam Antibiotics Using Sweat Analysis-A Pilot Study.Frontiers in medicine · 2020Article
- Single-Dose Pharmacokinetics and Preliminary Safety Assessment with Use of CBD-Rich Hemp Nutraceutical in Healthy Dogs and Cats.Animals : an open access journal from MDPI · 2019Article
- Impact of Wearing and Washing/Drying of Permethrin-Treated Clothing on Their Contact Irritancy and Toxicity for Nymphal Ixodes scapularis (Acari: Ixodidae) Ticks.Journal of medical entomology · 2019Article
- Pregnenolone-progesterone-allopregnanolone pathway as a potential therapeutic target in first-episode antipsychotic-naïve patients with schizophrenia.Psychoneuroendocrinology · 2018Article
- Metabolomics and Ionomics of Potato Tuber Reveals an Influence of Cultivar and Market Class on Human Nutrients and Bioactive Compounds.Frontiers in nutrition · 2018Article
- Pharmacokinetics, Safety, and Clinical Efficacy of Cannabidiol Treatment in Osteoarthritic Dogs.Frontiers in veterinary science · 2018Article
- Model of Chronic Equine Endometritis Involving a Pseudomonas aeruginosa Biofilm.Infection and immunity · 2017Article
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Simultaneous determination of cortisol and cortisone from human serum by liquid chromatography-tandem mass spectrometry.Journal of analytical methods in chemistry · 2014Article
- Hyphenation of microflow chromatography with electrospray ionization mass spectrometry for bioanalytical applications focusing on low molecular weight compounds: A tutorial review.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A novel microfluidic chromatography device coupled with tandem mass spectrometry (LC-MS/MS) was utilized for the multiplex analysis of 5 steroids (testosterone, dihydrotestosterone, progesterone, cortisol, cortisone) in human serum. The use of microfluidics allowed for reduction of the chromatographic flow rate to 3μl/min with overall method run times comparable to standard flow LC-MS/MS methods reported in the literature, corresponding to a 150 fold decrease in solvent consumption. Furthermore, a simple sample preparation protocol was employed requiring injection of only 0.5μl of sample, corresponding to a 100-400 fold increase in on-column sensitivity as compared to published standard flow assays. The measured LOQ for both testosterone and progesterone was 0.4ng/mL, representing an improvement over reported literature values obtained by standard flow methods employing comparable sample preparation and large injection volumes. The LOQs for cortisol (1.9ng/mL), cortisone (0.3ng/mL), and dihydrotestosterone (1.4ng/mL) were all within a biologically relevant range. A comparison of clinical serum samples was performed for the analysis of testosterone using this microfluidic LC-MS/MS assay and the Beckman Access II automated antibody-based measurement system. The immunoassay results were systematically higher due to matrix interference which was easily resolved with the increased chromatographic resolution obtained in the microflow LC-MS/MS assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.