ArticleCurrent biology : CB2013
Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 61 citations in OpenAlex.
- Transport and barrier mechanisms that regulate ciliary compartmentalization and ciliopathies.Nature reviews. Nephrology · 2024Review
- Regulation of ciliary homeostasis by intraflagellar transport-independent kinesins.Cell death & disease · 2024Review
- Determinants of cytoplasmic microtubule depolymerization during ciliogenesis inLife science alliance · 2024Article
- Initial ciliary assembly inMolecular biology of the cell · 2023Article
- Cargo adapters expand the transport range of intraflagellar transport.Journal of cell science · 2022Review
- Kinesin-2 motors differentially impact biogenesis of extracellular vesicle subpopulations shed from sensory cilia.iScience · 2022Article
- Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex.eLife · 2022Article
- Cilia proteins getting to work - how do they commute from the cytoplasm to the base of cilia?Journal of cell science · 2022Review
- A cytoplasmic protein kinase couples engagement of Chlamydomonas ciliary receptors to cAMP-dependent cellular responses.Journal of cell science · 2022Article
- MAR1 links membrane adhesion to membrane merger during cell-cell fusion in Chlamydomonas.Developmental cell · 2021Article
- The elusive actin cytoskeleton of a green alga expressing both conventional and divergent actins.Molecular biology of the cell · 2019Article
- Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium.Current biology : CB · 2019Article
- Establishing and regulating the composition of cilia for signal transduction.Nature reviews. Molecular cell biology · 2019Review
- Ciliotherapy: Remote Control of Primary Cilia Movement and Function by Magnetic Nanoparticles.ACS nano · 2019Article
- The molecular machines that traffic signaling receptors into and out of cilia.Current opinion in cell biology · 2018Review
- Protein transport in growing and steady-state cilia.Traffic (Copenhagen, Denmark) · 2017Review
- Article
- KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.Nature communications · 2017Article
- Trafficking to the primary cilium membrane.Molecular biology of the cell · 2017Review
- IFT-Cargo Interactions and Protein Transport in Cilia.Trends in biochemical sciences · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The membrane protein composition of the primary cilium, a key sensory organelle, is dynamically regulated during cilium-generated signaling [1, 2]. During ciliogenesis, ciliary membrane proteins, along with structural and signaling proteins, are carried through the multicomponent, intensely studied ciliary diffusion barrier at the base of the organelle [3-8] by intraflagellar transport (IFT) [9-18]. A favored model is that signaling-triggered accumulation of previously excluded membrane proteins in fully formed cilia [19-21] also requires IFT, but direct evidence is lacking. Here, in studies of regulated entry of a membrane protein into the flagellum of Chlamydomonas, we show that cells use an IFT-independent mechanism to breach the diffusion barrier at the flagellar base. In resting cells, a flagellar signaling component [22], the integral membrane polypeptide SAG1-C65, is uniformly distributed over the plasma membrane and excluded from the flagellar membrane. Flagellar adhesion-induced signaling triggers rapid, striking redistribution of the protein to the apical ends of the cells concomitantly with entry into the flagella. Protein polarization and flagellar enrichment are facilitated by cytoplasmic microtubules. Using a conditional anterograde IFT mutant, we demonstrate that the IFT machinery is not required for regulated SAG1-C65 entry into flagella. Thus, integral membrane proteins can negotiate passage through the ciliary diffusion barrier without the need for a motor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.