ArticleMalaria journal2013
Prevalence of drug resistance associated mutations in Plasmodium vivax against sulphadoxine-pyrimethamine in southern Pakistan.
Article in Malaria journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Surveillance of molecular markers of antimalarial drug resistance in Plasmodium falciparum and Plasmodium vivax in Federally Administered Tribal Area (FATA), Pakistan.Revista do Instituto de Medicina Tropical de Sao Paulo · 2021Article
- Polymorphisms in Plasmodium vivax antifolate resistance markers in Afghanistan between 2007 and 2017.Malaria journal · 2020Article
- Genetic diversity and transmissibility of imported Plasmodium vivax in Qatar and three countries of origin.Scientific reports · 2018Article
- Aptamer Technology: Adjunct Therapy for Malaria.Biomedicines · 2017Review
- Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia.Malaria journal · 2016Article
- Article
- Whole Genome Sequencing of Field Isolates Reveals Extensive Genetic Diversity in Plasmodium vivax from Colombia.PLoS neglected tropical diseases · 2015Article
- Vivax malaria and chloroquine resistance: a neglected disease as an emerging threat.Malaria journal · 2015Article
- Epidemiology, drug resistance, and pathophysiology ofJournal of vector borne diseasesReview
- Allelic Variations ofIranian journal of parasitologyArticle
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Abstract
backgroundIn Pakistan, Plasmodium vivax and Plasmodium falciparum co-exist and usage of sulphadoxine-pyrimethamine (SP) against P. falciparum exposes P. vivax to the drug leading to generation of resistant alleles. The main aim of this study was to investigate frequency distribution of drug resistance associated mutations in pvdhfr, pvdhps genes and provide baseline molecular epidemiological data on SP-associated resistance in P. vivax from southern Pakistan.
methodsFrom January 2008 to May 2009, a total of 150 samples were collected from patients tested slide-positive for P. vivax, at the Aga Khan University Hospital, Karachi, or its collection units located in Baluchistan and Sindh Province. Nested PCR using pvdhfr and pvdhps specific primers was performed for all samples.91.3% (137/150) of the samples were tested PCR positive of which 87.3% (131/137) were successfully sequenced. Sample sequencing data was analysed and compared against wild type reference sequences.
resultsIn dhfr, mutations were observed at codons F57L, S58R and S117N/T. Novel non-synonymous mutations were observed at codon positions N50I, G114R and E119K while a synonymous mutation was observed at codon position 69Y. In dhps, mutations were observed at codon position A383G and A553G while novel non-synonymous mutations were observed at codon positions S373T, E380K, P384L, N389T, V392D, T393P, D459A, M601I, A651D and A661V.
conclusionThis is the first report from southern Pakistan on SP resistance in clinical isolates of P. vivax. Results from this study confirm that diverse drug resistant alleles are circulating within this region.
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