Evidence map›Paper›PMID 23890073›Full record

ArticleBiotechnology for biofuels2013

Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover.

Mingjie Jin, Cory Sarks, Christa Gunawan, Benjamin D Bice, Shane P Simonett, Ragothaman Avanasi Narasimhan, Laura B Willis, Bruce E Dale, Venkatesh Balan, Trey K Sato

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
5.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 52 citations in OpenAlex.

  1. Review
  2. Metabolic engineering ofBiotechnology for biofuels · 2017
    Article
  3. Hybridization and adaptive evolution of diverseBiotechnology for biofuels · 2017
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Mingjie JinBiomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA ; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Cory SarksBiomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA ; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Christa GunawanBiomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA ; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Benjamin D BiceDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave, Madison, WI 53726, USA.
Shane P SimonettDepartment of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI 53706, USA.
Ragothaman Avanasi NarasimhanDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave, Madison, WI 53726, USA.
Laura B WillisDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave, Madison, WI 53726, USA ; Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI 53706, USA ; U.S. Department of Agriculture, Forest Products Laboratory, 1 Gifford Pinchot Dr, Madison, WI 53726, USA.
Bruce E DaleBiomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA ; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Venkatesh BalanBiomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA ; DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Trey K SatoDOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1552 University Ave, Madison, WI 53726, USA.
Great Lakes Bioenergy Research Center · USMichigan State University · USUniversity of Wisconsin–Madison · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSimultaneous saccharification and co-fermentation (SSCF) process involves enzymatic hydrolysis of pretreated lignocellulosic biomass and fermentation of glucose and xylose in one bioreactor. The optimal temperatures for enzymatic hydrolysis are higher than the standard fermentation temperature of ethanologenic Saccharomyces cerevisiae. Moreover, degradation products resulting from biomass pretreatment impair fermentation of sugars, especially xylose, and can synergize with high temperature stress. One approach to resolve both concerns is to utilize a strain background with innate tolerance to both elevated temperatures and degradation products.

resultsIn this study, we screened a panel of 108 wild and domesticated Saccharomyces cerevisiae strains isolated from a wide range of environmental niches. One wild strain was selected based on its growth tolerance to simultaneous elevated temperature and AFEX™ (Ammonia Fiber Expansion) degradation products. After engineering the strain with two copies of the Scheffersomyces stipitis xylose reductase, xylitol dehydrogenase and xylulokinase genes, we compared the ability of this engineered strain to the benchmark 424A(LNH-ST) strain in ethanol production and xylose fermentation in standard lab medium and AFEX pretreated corn stover (ACS) hydrolysates, as well as in SSCF of ACS at different temperatures. In SSCF of 9% (w/w) glucan loading ACS at 35°C, the engineered strain showed higher cell viabilities and produced a similar amount of ethanol (51.3 g/L) compared to the benchmark 424A(LNH-ST) strain.

conclusionThese results validate our approach in the selection of wild Saccharomyces cerevisiae strains with thermo-tolerance and degradation products tolerance properties for lignocellulosic biofuel production. The wild and domesticated yeast strains phenotyped in this work are publically available for others to use as genetic backgrounds for fermentation of their pretreated biomass at elevated temperatures.

Indexed as

AFEXDegradation productsEthanolS. cerevisiaeSSCFThermo-toleranceXylose fermentation

Identifiers

PMID23890073
PMCPMC3729497
OpenAlexW2132656684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.