ArticleNeuron2013
Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI).
Article in Neuron, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 1 of them a synthesis that pooled it.
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Who cites it
91 citing papers in PubMed, 1 synthesis or guideline pooled it, 182 citations in OpenAlex.
- Effects of Light on Attention and Reaction Time: A Systematic Review.Journal of research in health sciences · 2021Pooled it
- Light-activated tetanus neurotoxin for conditional proteolysis and inducible synaptic inhibition in vivo.Nature methods · 2026Article
- Cerebellar neural populations orchestrate dopamine reward signaling with single-trial precision.Nature communications · 2026Article
- Modulating Protein Function through Genetically Encoded Oxidative Chemistry.Journal of the American Chemical Society · 2026Article
- Functional interrogation of neuronal connections by chemoptogenetic presynaptic ablation.Science advances · 2026Article
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
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- C-LTMRs evoke wet dog shakes via the spinoparabrachial pathway.Science (New York, N.Y.) · 2024Article
- Chromophore-Assisted Light Inactivation for Protein Degradation and Its Application in Biomedicine.Bioengineering (Basel, Switzerland) · 2024Review
- Cellular and subcellular optogenetic approaches towards neuroprotection and vision restoration.Progress in retinal and eye research · 2023Review
- Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome.Nature communications · 2023Article
- Article
- Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles.Nature communications · 2022Article
- Optogenetic polymerization and assembly of electrically functional polymers for modulation of single-neuron excitability.Science advances · 2022Article
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Article
- A Photosensitized Singlet Oxygen (Molecules (Basel, Switzerland) · 2022Review
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- A variety of photoreceptors and the frontiers of optogenetics.Biophysics and physicobiology · 2022Article
31 more citing papers are in PubMed but not listed here.
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8 authors at 2 institutions in 1 country.
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Abstract
Optogenetic techniques provide effective ways of manipulating the functions of selected neurons with light. In the current study, we engineered an optogenetic technique that directly inhibits neurotransmitter release. We used a genetically encoded singlet oxygen generator, miniSOG, to conduct chromophore assisted light inactivation (CALI) of synaptic proteins. Fusions of miniSOG to VAMP2 and synaptophysin enabled disruption of presynaptic vesicular release upon illumination with blue light. In cultured neurons and hippocampal organotypic slices, synaptic release was reduced up to 100%. Such inhibition lasted >1 hr and had minimal effects on membrane electrical properties. When miniSOG-VAMP2 was expressed panneuronally in Caenorhabditis elegans, movement of the worms was reduced after illumination, and paralysis was often observed. The movement of the worms recovered overnight. We name this technique Inhibition of Synapses with CALI (InSynC). InSynC is a powerful way to silence genetically specified synapses with light in a spatially and temporally precise manner.
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