ArticleBMC complementary and alternative medicine2013
Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2.
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- ANTI-VIRAL EFFECTS OF MEDICINAL PLANTS IN THE MANAGEMENT OF DENGUE: A SYSTEMATIC REVIEW.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2017Pooled it
- Unlocking the Therapeutic Potential of Ellagitannins: A Comprehensive Review of Key Representatives.Molecules (Basel, Switzerland) · 2025Review
- Identification of Potential Inhibitors Targeting Non-Structural Proteins NS3 and NS5 of Dengue Virus Using Docking and Deep Learning Approaches.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Exploring plant-based dengue therapeutics: from laboratory to clinic.Tropical diseases, travel medicine and vaccines · 2024Article
- Medicinal plants mediated the green synthesis of silver nanoparticles and their biomedical applications.IET nanobiotechnology · 2022Review
- Multidrug resistance from a one health perspective in Ethiopia: A systematic review and meta-analysis of literature (2015-2020).One health (Amsterdam, Netherlands) · 2022Review
- Phytoconstituents as Lead Compounds for Anti-Dengue Drug Discovery.Advances in experimental medicine and biology · 2021Article
- Production, Transmission, Pathogenesis, and Control of Dengue Virus: A Literature-Based Undivided Perspective.BioMed research international · 2021Review
- Potential Medicinal Plants for the Treatment of Dengue Fever and Severe Acute Respiratory Syndrome-Coronavirus.Biomolecules · 2020Review
- Antiviral Role of Phenolic Compounds against Dengue Virus: A Review.Biomolecules · 2020Review
- In-vitro and in silico activity ofVirusdisease · 2020Article
- Pressurized hot water extraction of hydrosable tannins fromBMC chemistry · 2019Article
- In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting.BMC complementary and alternative medicine · 2019Article
- Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.Scientific reports · 2019Article
- Efficacy of geraniin on dengue virus type-2 infected BALB/c mice.Virology journal · 2019Article
- A Review of the Phytochemistry and Pharmacology ofFrontiers in pharmacology · 2018Article
- Geraniin extracted from the rind of Nephelium lappaceum binds to dengue virus type-2 envelope protein and inhibits early stage of virus replication.Virology journal · 2017Article
- Papaya extract to treat dengue: a novel therapeutic option?Annals of medical and health sciences research · 2014Review
- Inhibition of Raf-MEK-ERK and hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line.BMC complementary and alternative medicine · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe absence of commercialized vaccines and antiviral agents against dengue has made the disease a major health concern around the world. With the current dengue virus transmission rate and incidences, the development of antiviral drugs is of vital need. The aim of this project was to evaluate the possibility of developing a local medicinal plant, Phyllanthus as an anti-dengue agent.
methodsCocktail (aqueous and methanolic) extracts were prepared from four species of Phyllanthus (P.amarus, P.niruri, P.urinaria, and P.watsonii) and their polyphenolic compounds were identified via HPLC and LC-MS/MS analysis. MTS assay was then carried out to determine the maximal non-toxic dose (MNTD) of the extracts, followed by screening of the in vitro antiviral activity of aqueous cocktail extracts against DENV2 by means of time-of-addition (pre-, simultaneous and post-) using RT-qPCR. The differentially expressed proteins in the treated and infected cells were analysed with two dimensional gel electrophoresis experiments.
resultsSeveral active compounds including gallic acid, geraniin, syringin, and corilagen have been identified. The MNTD of both aqueous and methanolic extracts on Vero cells were 250.0 μg/ml and 15.63 μg/ml respectively. Phyllanthus showed strongest inhibitory activity against DENV2 with more than 90% of virus reduction in simultaneous treatment. Two-dimensional analysis revealed significantly altered levels of thirteen proteins, which were successfully identified by tandem MS (MS/MS). These altered proteins were involved in several biological processes, including viral entry, viral transcription and translation regulations, cytoskeletal assembly, and cellular metabolisms.
conclusionsPhyllanthus could be potentially developed as an anti-DENV agent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.