ArticleJournal of assisted reproduction and genetics2013
A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- The Presence of TGFβ3 in Human Ovarian Intrafollicular Fluid and Its Involvement in Thromboxane Generation in Follicular Granulosa Cells through a Canonical TGFβRI, Smad2/3 Signaling Pathway and COX-2 Induction.International journal of molecular sciences · 2024Article
- Multi-omics approach to reveal follicular metabolic changes and their effects on oocyte competence in PCOS patients.Frontiers in endocrinology · 2024Article
- High coverage of targeted lipidomics revealed lipid changes in the follicular fluid of patients with insulin-resistant polycystic ovary syndrome and a positive correlation between plasmalogens and oocyte quality.Frontiers in endocrinology · 2024Article
- A Proteomic Analysis of Human Follicular Fluid: Proteomic Profile Associated with Embryo Quality.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- A Case-Control Study of Follicular Fluid Cytokine Profiles in Women with Diminished Ovarian Reserve.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Implementing a preimplantation proteomic approach to advance assisted reproduction technologies in the framework of predictive, preventive, and personalized medicine.The EPMA journal · 2022Review
- The Effects of SARS-CoV-2 Infection on Female Fertility: A Review of the Literature.International journal of environmental research and public health · 2022Review
- Identification of the Metabolomics Signature of Human Follicular Fluid from PCOS Women with Insulin Resistance.Disease markers · 2022Article
- Ovarian follicular function is not altered by SARS-CoV-2 infection or BNT162b2 mRNA COVID-19 vaccination.Human reproduction (Oxford, England) · 2021Article
- Compatibility of Distinct Label-Free Proteomic Workflows in Absolute Quantification of Proteins Linked to the Oocyte Quality in Human Follicular Fluid.International journal of molecular sciences · 2021Article
- Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.Human reproduction (Oxford, England) · 2021Article
- Fatty acid induced lipolysis influences embryo development, gene expression and lipid droplet formation in the porcine cumulus cells†.Biology of reproduction · 2020Article
- Comparative Analysis of Porcine Follicular Fluid Proteomes of Small and Large Ovarian Follicles.Biology · 2020Article
- ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes.Journal of assisted reproduction and genetics · 2020Article
- Proteome changes of porcine follicular fluid during follicle development.Journal of animal science and biotechnology · 2019Article
- Proteomic analysis of human follicular fluid associated with successful in vitro fertilization.Reproductive biology and endocrinology : RB&E · 2017Article
- Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success.Journal of assisted reproduction and genetics · 2016Article
- Follicular fluid biomarkers for human in vitro fertilization outcome: Proof of principle.Proteome science · 2016Article
- CYP51A1 induced by growth differentiation factor 9 and follicle-stimulating hormone in granulosa cells is a possible predictor for unfertilization.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHuman follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization.
methodWe performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses.
resultsWe identified a total of 503 proteins in human follicular fluids containing fertilized and non-fertilized oocytes obtained from 12 patients. We also found that 53 proteins exhibited significantly different spectral counts between the two groups, including heparan sulfate proteoglycan perlecan, which showed significant upregulation in the follicular fluids containing fertilized oocytes in comparison with that observed in the follicular fluids containing non-fertilized oocytes.
conclusionOur results suggest a possibility that proteins identified by LC/MS/MS in follicular fluid might not only be involved in folliculogenesis, but also function as biomarkers possessing predictive potential for oocyte maturation and the success of IVF when their expression levels are significantly different between fertilized and non-fertilized oocytes, although no distinctive biomarkers were identified in the current study.
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Registered trials
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