ArticleNucleic acids research1990
A novel transcriptional activator (PO-B) binds between the TATA box and cap site of the pro-opiomelanocortin gene.
Article in Nucleic acids research, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- The human POMC gene promoter: where do we stand?Journal of endocrinological investigation · 2011Review
- Role of transcription factors in the pathogenesis of pituitary adenomas: a review.Journal of neuro-oncology · 2001Review
- Subtle determinants of the nucleocytoplasmic partitioning of in vivo-transcribed RNase MRP RNA in Xenopus laevis oocytes.Gene expression · 1996Article
- DNA elements with AT-rich core sequences direct pituitary cell-specific expression of the pro-opiomelanocortin gene in transgenic mice.The Biochemical journal · 1995Article
- Lens-specific activity of the mouse alpha A-crystallin promoter in the absence of a TATA box: functional and protein binding analysis of the mouse alpha A-crystallin PE1 region.Nucleic acids research · 1995Article
- Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter.The Journal of clinical investigation · 1994Article
- Cell-specific helix-loop-helix factor required for pituitary expression of the pro-opiomelanocortin gene.Molecular and cellular biology · 1993Article
- Identification of DNA elements cooperatively activating proopiomelanocortin gene expression in the pituitary glands of transgenic mice.Molecular and cellular biology · 1992Article
- Pituitary pro-opiomelanocortin gene expression requires synergistic interactions of several regulatory elements.Molecular and cellular biology · 1991Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate that a factor (PO-B), detected in a number of mammalian cell lines, binds specifically between the TATA box and the transcription initiation site of the pituitary-specific pro-opiomelanocortin (POMC) gene. Methylation interference and DNAse 1 footprint analysis revealed that the binding site of this protein, -3 to -15, does not overlap the POMC TATA box or cap site. Mutation of the PO-B binding site significantly decreased the transcriptional activity of the POMC promoter after transient transfection into the ATt-20 mouse pituitary tumor cell line and also in in vitro transcription assays. In contrast, mutation of the TATA box produced no overall decrease in transcription in vivo but induced multiple heterogeneous start sites in vitro. A vector harboring the PO-B site alone was unable to direct transcription initiation. PO-B represents a new transcription factor that may be able to facilitate POMC gene expression by interacting with components of the transcription initiation complex.
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