ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2013
Comparison of histological techniques to visualize iron in paraffin-embedded brain tissue of patients with Alzheimer's disease.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 61 citations in OpenAlex.
- MatchingFrontiers in neuroanatomy · 2019Pooled it
- Markers of microglia in post-mortem brain samples from patients with Alzheimer's disease: a systematic review.Molecular psychiatry · 2018Pooled it
- T1-weighted/T2-weighted ratio reflects microstructural changes in Alzheimer's disease.Alzheimer's research & therapy · 2026Article
- Reexamining the Role of Amyloid β Clearance from the Brain: Exporting Labile Iron from the Interstitial Fluid Performs a Protective Function.International journal of molecular sciences · 2026Review
- Clinicoanatomic localization of iron-rich gliosis in aphasic presentations of globular glial tauopathy.Brain communications · 2026Article
- Perls' Prussian blue staining and chemistry of Prussian blue and Turnbull blue.Forensic science international. Synergy · 2025Review
- Effects of iron accumulation and its chelation on oxidative stress in intracortical implants.Acta biomaterialia · 2025Article
- Exploring Iron Deposition Patterns Using Light and Electron Microscopy in the Mouse Brain Across Aging and Alzheimer's Disease Pathology Conditions.Journal of neurochemistry · 2025Article
- Iron-associated lipid peroxidation in Alzheimer's disease is increased in lipid rafts with decreased ferroptosis suppressors, tested by chelation in mice.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- The Azalea Hypothesis of Alzheimer Disease: A Functional Iron Deficiency Promotes Neurodegeneration.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2024Review
- Ethanol Exacerbates the Alzheimer's Disease Pathology in the 5xFAD Mouse Model.Neuroglia (Basel, Switzerland) · 2024Article
- A commentary on studies of brain iron accumulation during ageing.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2024Review
- Iron Dysregulation in Alzheimer's Disease: LA-ICP-MS Bioimaging of the Distribution of Iron and Ferroportin in the CA1 Region of the Human Hippocampus.Biomolecules · 2024Article
- Metallothionein I/II Expression and Metal Ion Levels in Correlation with Amyloid Beta Deposits in the Aged Feline Brain.Brain sciences · 2023Article
- Cortical iron accumulation in MAPT- and C9orf 72-associated frontotemporal lobar degeneration.Brain pathology (Zurich, Switzerland) · 2023Article
- Complement Drives Chronic Inflammation and Progressive Hydrocephalus in Murine Neonatal Germinal Matrix Hemorrhage.International journal of molecular sciences · 2023Article
- Insertable inductively coupled volumetric coils for MR microscopy in a human 7T MR system.Magnetic resonance in medicine · 2022Article
- Off-resonance saturation as an MRI method to quantify mineral- iron in the post-mortem brain.Magnetic resonance in medicine · 2022Article
- Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains.PloS one · 2022Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Better knowledge of the distribution of iron in the brains of Alzheimer's disease (AD) patients may facilitate the development of an in vivo magnetic resonance (MR) marker for AD and may cast light on the role of this potentially toxic molecule in the pathogenesis of AD. Several histological iron staining techniques have been used in the past but they have not been systematically tested for sensitivity and specificity. This article compares three histochemical techniques and ferritin immunohistochemistry to visualize iron in paraffin-embedded human AD brain tissue. The specificity of the histochemical techniques was tested by staining sections after iron extraction. Iron was demonstrated in the white matter, in layers IV/V of the frontal neocortex, in iron containing plaques, and in microglia. In our hands, these structures were best visualized using the Meguro iron stain, a method that has not been described for iron staining in human brain or AD in particular. Ferritin immunohistochemistry stained microglia and iron containing plaques similar to the Meguro method but was less intense in myelin-associated iron. The Meguro method is most suitable for identifying iron-positive structures in paraffin-embedded human AD brain tissue.
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