ArticleCritical care medicine2013
Adverse effects of hemorrhagic shock resuscitation with stored blood are ameliorated by inhaled nitric oxide in lambs*.
Article in Critical care medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Cancer- and cardiac-induced cachexia: same fate through different inflammatory mediators?Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022Pooled it
- Older Blood Is Associated With Increased Mortality and Adverse Events in Massively Transfused Trauma Patients: Secondary Analysis of the PROPPR Trial.Annals of emergency medicine · 2019Trial
- Trial
- Effects of Aged Stored Autologous Red Blood Cells on Human Endothelial Function.American journal of respiratory and critical care medicine · 2015Trial
- Autologous transfusion of stored red blood cells increases pulmonary artery pressure.American journal of respiratory and critical care medicine · 2014Trial
- Storage duration of packed red blood cells transfused during veno-venous extracorporeal membrane oxygenation is associated with elevated pulmonary artery pressure and lung injury in a sheep model.Critical care (London, England) · 2025Article
- Large Animal Models for Simulating Physiology of Transfusion of Red Cell Concentrates-A Scoping Review of The Literature.Medicina (Kaunas, Lithuania) · 2022Article
- Nitric oxide: Clinical applications in critically ill patients.Nitric oxide : biology and chemistry · 2022Review
- Inhaled nitric oxide.British journal of pharmacology · 2019Review
- Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study.PLoS medicine · 2018Article
- Parenteral irons versus transfused red blood cells for treatment of anemia during canine experimental bacterial pneumonia.Transfusion · 2017Article
- Risk Factors and Clinical Outcomes Associated with Perioperative Transfusion-associated Circulatory Overload.Anesthesiology · 2017Article
- Exposure of Stored Packed Erythrocytes to Nitric Oxide Prevents Transfusion-associated Pulmonary Hypertension.Anesthesiology · 2016Article
- Haptoglobin or Hemopexin Therapy Prevents Acute Adverse Effects of Resuscitation After Prolonged Storage of Red Cells.Circulation · 2016Article
- In a canine pneumonia model of exchange transfusion, altering the age but not the volume of older red blood cells markedly alters outcome.Transfusion · 2015Article
- Transfused older stored red blood cells improve the clinical course and outcome in a canine lethal hemorrhage and reperfusion model.Transfusion · 2015Article
- Perioperative Red Blood Cell Transfusion: What We Do Not Know.Chinese medical journal · 2015Review
- Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage.Lab on a chip · 2015Article
- Our paper 20 years later: Inhaled nitric oxide for the acute respiratory distress syndrome--discovery, current understanding, and focussed targets of future applications.Intensive care medicine · 2014Article
- Dose-Dependent Hemodynamic, Biochemical, and Tissue Oxygen Effects of OC99 following Severe Oxygen Debt Produced by Hemorrhagic Shock in Dogs.Critical care research and practice · 2014Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
objectivesTransfusion of stored RBCs is associated with increased morbidity and mortality in trauma patients. Plasma hemoglobin scavenges nitric oxide, which can cause vasoconstriction, induce inflammation, and activate platelets. We hypothesized that transfusion of RBCs stored for prolonged periods would induce adverse effects (pulmonary vasoconstriction, tissue injury, inflammation, and platelet activation) in lambs subjected to severe hemorrhagic shock and that concurrent inhalation of nitric oxide would prevent these adverse effects.
designAnimal study.
settingResearch laboratory at the Massachusetts General Hospital, Boston, MA. SUBJECTS: Seventeen awake Polypay-breed lambs.
interventionsLambs were subjected to 2 hours of hemorrhagic shock by acutely withdrawing 50% of their blood volume. Lambs were resuscitated with autologous RBCs stored for 2 hours or less (fresh) or 39 ± 2 (mean ± SD) days (stored). Stored RBCs were administered with or without breathing nitric oxide (80 ppm) during resuscitation and for 21 hours thereafter. MEASUREMENTS AND MAIN
resultsWe measured hemodynamic and oxygenation variables, markers of tissue injury and inflammation, plasma hemoglobin concentrations, and platelet activation. Peak pulmonary arterial pressure was higher after resuscitation with stored than with fresh RBCs (24 ± 4 vs 14 ± 2 mm Hg, p < 0.001) and correlated with peak plasma hemoglobin concentrations (R = 0.56, p = 0.003). At 21 hours after resuscitation, pulmonary myeloperoxidase activity was higher in lambs resuscitated with stored than with fresh RBCs (11 ± 2 vs 4 ± 1 U/g, p = 0.007). Furthermore, transfusion of stored RBCs increased plasma markers of tissue injury and sensitized platelets to adenosine diphosphate activation. Breathing nitric oxide prevented the pulmonary hypertension and attenuated the pulmonary myeloperoxidase activity, as well as tissue injury and sensitization of platelets to adenosine diphosphate.
conclusionsOur data suggest that resuscitation of lambs from hemorrhagic shock with autologous stored RBCs induces pulmonary hypertension and inflammation, which can be ameliorated by breathing nitric oxide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.