ArticleScientific reports2013
Whole-cell phase contrast imaging at the nanoscale using Fresnel coherent diffractive imaging tomography.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Ptychographic imaging of NaD1 induced yeast cell death.Biomedical optics express · 2019Article
- A comparison of absorption and phase contrast for X-ray imaging of biological cells.Journal of synchrotron radiation · 2018Article
- Lensless Tomographic Imaging of Near Surface Structures of Frozen Hydrated Malaria-Infected Human Erythrocytes by Coherent X-Ray Diffraction Microscopy.Scientific reports · 2017Article
- Laboratory cryo x-ray microscopy for 3D cell imaging.Scientific reports · 2017Article
- Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells.Scientific reports · 2016Article
- Ptychographic Imaging of Branched Colloidal Nanocrystals Embedded in Free-Standing Thick Polystyrene Films.Scientific reports · 2016Article
- Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging.Biophysical journal · 2015Article
- Quantitative X-ray phase contrast waveguide imaging of bacterial endospores.Journal of applied crystallography · 2015Article
- Coherent X-ray diffraction imaging and characterization of strain in silicon-on-insulator nanostructures.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- Mapping biological composition through quantitative phase and absorption X-ray ptychography.Scientific reports · 2014Article
- Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy.Biophysical journal · 2014Article
- Coherent imaging at the diffraction limit.Journal of synchrotron radiation · 2014Article
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14 authors.
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Abstract
X-ray tomography can provide structural information of whole cells in close to their native state. Radiation-induced damage, however, imposes a practical limit to image resolution, and as such, a choice between damage, image contrast, and image resolution must be made. New coherent diffractive imaging techniques, such Fresnel Coherent Diffractive Imaging (FCDI), allows quantitative phase information with exceptional dose efficiency, high contrast, and nano-scale resolution. Here we present three-dimensional quantitative images of a whole eukaryotic cell by FCDI at a spatial resolution below 70 nm with sufficient phase contrast to distinguish major cellular components. From our data, we estimate that the minimum dose required for a similar resolution is close to that predicted by the Rose criterion, considerably below accepted estimates of the maximum dose a frozen-hydrated cell can tolerate. Based on the dose efficiency, contrast, and resolution achieved, we expect this technique will find immediate applications in tomographic cellular characterisation.
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