ArticleNature protocols2013
Design and generation of recombinant rabies virus vectors.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 172 papers, 1 of them a synthesis that pooled it.
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Who cites it
172 citing papers in PubMed, 1 synthesis or guideline pooled it, 305 citations in OpenAlex.
- A Systematic Review of Direct Outputs from the Cerebellum to the Brainstem and Diencephalon in Mammals.Cerebellum (London, England) · 2024Pooled it
- Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq.Cell reports methods · 2026Article
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Integrated 5-HTMolecular psychiatry · 2026Article
- High ambient temperature activates a neural circuit for gut glucose uptake in male mice.Nature communications · 2026Article
- Nos1 neurons in the paraventricular hypothalamic area modulate lipid metabolism via the sympathetic nervous system in male mice.Nature communications · 2026Article
- Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice.STAR protocols · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- GABAergic Inhibition from the Nucleus Tractus Solitarius to Ventrolateral Medulla Phox2b Neurons Modulates Central Respiratory Chemoreflex and Ventilatory Homeostasis.Neuroscience bulletin · 2026Article
- Engineering multiple levels of specificity in an RNA viral vector.Nature communications · 2026Article
- Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice.iScience · 2026Article
- Development of the reverse genetics system for viral hemorrhagic septicemia virus genotype IVa and its application in antiviral compound screening.Virologica Sinica · 2026Article
- Ngn2 and Isl1-mediated astrocyte-to-neuron conversion in vivo promotes functional recovery after spinal cord injury.Cell reports. Medicine · 2025Article
- Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons.Cell reports methods · 2025Article
- One-step approach producing barcoded rabies virus with optimized diversity.Cell reports methods · 2025Article
- The Bed Nucleus of the Stria Terminalis-Deep Mesencephalic Nucleus Circuit Linking Emotion and Wakefulness.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Vasopressin-to-oxytocin receptor crosstalk in the preoptic area underlying parental behaviors in male mice.Nature communications · 2025Article
- Thalamus-cortex interactions drive cell type-specific cortical development in human pluripotent stem cell-derived assembloids.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
112 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rabies viruses, negative-strand RNA viruses, infect neurons through axon terminals and spread trans-synaptically in a retrograde direction between neurons. Rabies viruses whose glycoprotein (G) gene is deleted from the genome cannot spread across synapses. Complementation of G in trans, however, enables trans-synaptic spreading of G-deleted rabies viruses to directly connected, presynaptic neurons. Recombinant rabies viruses can encode genes of interest for labeling cells, controlling gene expression and monitoring or manipulating neural activity. Cre-dependent or bridge protein-mediated transduction and single-cell electroporation via the EnvA-TVA or EnvB-TVB (envelope glycoprotein and its specific receptor for avian sarcoma leukosis virus subgroup A or B) system allow cell type-specific or single cell-specific targeting. These rabies virus-based approaches permit the linking of connectivity to cell morphology and circuit function for particular cell types or single cells. Here we describe methods for construction of rabies viral vectors, recovery of G-deleted rabies viruses from cDNA, amplification of the viruses, pseudotyping them with EnvA or EnvB and concentration and titration of the viruses. The entire protocol takes 6-8 weeks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.