ArticleMolecular and cellular biology1990
Overlapping reading frames at the LYS5 locus in the yeast Yarrowia lipolytica.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 52 citations in OpenAlex.
- Non-homologous End Joining-Mediated Insertional Mutagenesis Reveals a Novel Target for Enhancing Fatty Alcohols Production inFrontiers in microbiology · 2022Article
- Application of a New Engineered Strain ofInternational journal of molecular sciences · 2021Article
- Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study.Biotechnology for biofuels · 2021Article
- Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions.Microbial cell factories · 2019Article
- Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica.Biotechnology for biofuels · 2015Article
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
- Functional analysis through site-directed mutations and phylogeny of the Candida albicans LYS1-encoded saccharopine dehydrogenase.Molecular genetics and genomics : MGG · 2006Article
- Autocloning and amplification of LIP2 in Yarrowia lipolytica.Applied and environmental microbiology · 2000Article
- A rac homolog is required for induction of hyphal growth in the dimorphic yeast Yarrowia lipolytica.Journal of bacteriology · 2000Article
- Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica.Journal of bacteriology · 2000Article
- Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica.Journal of bacteriology · 1999Article
- Lysine-ketoglutarate reductase and saccharopine dehydrogenase from Arabidopsis thaliana: nucleotide sequence and characterization.Plant molecular biology · 1997Article
- HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica.Molecular and cellular biology · 1997Article
- The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.Current genetics · 1996Article
- Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue.Current genetics · 1995Article
- Molecular and functional analysis of the LYS1 gene of Candida albicans.Infection and immunity · 1994Article
- A phosphatidylinositol/phosphatidylcholine transfer protein is required for differentiation of the dimorphic yeast Yarrowia lipolytica from the yeast to the mycelial form.The Journal of cell biology · 1994Article
- Two upstream activation sequences control the expression of the XPR2 gene in the yeast Yarrowia lipolytica.Molecular and cellular biology · 1994Article
- Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase.Current genetics · 1992Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutants affected at the LYS5 locus of Yarrowia lipolytica lack detectable dehydrogenase (SDH) activity. The LYS5 gene has previously been cloned, and we present here the sequence of the 2.5-kilobase-pair (kb) DNA fragment complementing the lys5 mutation. Two large antiparallel open reading frames (ORF1 and ORF2) were observed, flanked by potential transcription signals. Both ORFs appear to be transcribed, but several lines of evidence suggest that only ORF2 is translated and encodes SDH. (i) The global amino acid compositions of Saccharomyces cerevisiae SDH and of the putative ORF2 product are similar and that of ORF1 is dissimilar. (ii) An in-frame translational fusion of ORF2 with the Escherichia coli lacZ gene was introduced into yeast cells and resulted in a beta-galactosidase activity regulated similarly to SDH; no beta-galactosidase activity was obtained with an in-frame fusion of ORF1 with lacZ. (iii) The introduction of a stop codon at the beginning of ORF2 prevented SDH expression in yeast cells, whereas no phenotypic effect was observed when ORF1 translation was blocked.
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