ArticleMicrobial cell factories2013
Metabolic engineering of Bacillus subtilis for growth on overflow metabolites.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Enhanced extracellular respiration of engineered Bacillus subtilis via anodic electro-fermentation with pH optimisation.Biotechnology for biofuels and bioproducts · 2026Article
- Metabolic Engineering Strategy forFoods (Basel, Switzerland) · 2025Review
- Article
- Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria.Journal of bacteriology · 2025Review
- The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes.Microbial cell factories · 2024Article
- Design of a sorbitol-activated nitrogen metabolism-dependent regulatory system for redirection of carbon metabolism flow in Bacillus licheniformis.Nucleic acids research · 2023Article
- Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals.Microbial cell factories · 2023Article
- Biotechnological and food synthetic biology potential of platform strain:Synthetic and systems biotechnology · 2023Review
- Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
- Path to improving the life cycle and quality of genome-scale models of metabolism.Cell systems · 2021Review
- Isolation and Purification of a New Bacillus Subtilis Strain from Deer Dung with Anti-microbial and Anti-cancer Activities.Current medical science · 2021Article
- Efficient Genome Editing inMicroorganisms · 2020Article
- Towards the Anaerobic Production of Surfactin UsingFrontiers in bioengineering and biotechnology · 2020Article
- Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks.Journal of biological engineering · 2017Article
- The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.Microbial cell factories · 2016Review
- Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin.Applied microbiology and biotechnology · 2015Article
- Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis.Microbial cell factories · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genome of the important industrial host Bacillus subtilis does not encode the glyoxylate shunt, which is necessary to utilize overflow metabolites, like acetate or acetoin, as carbon source. In this study, the operon encoding the isocitrate lyase (aceB) and malate synthase (aceA) from Bacillus licheniformis was transferred into the chromosome of B. subtilis. The resulting strain was examined in respect to growth characteristics and qualities as an expression host.
resultsOur results show that the modified B. subtilis strain is able to grow on the C2 compound acetate. A combined transcript, protein and metabolite analysis indicated a functional expression of the native glyoxylate shunt of B. lichenifomis in B. subtilis. This metabolically engineered strain revealed better growth behavior and an improved activity of an acetoin-controlled expression system.
conclusionsThe glyoxylate shunt of B. licheniformis can be functionally transferred to B. subtilis. This novel strain offers improved properties for industrial applications, such as growth on additional carbon sources and a greater robustness towards excess glucose feeding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.