ArticleMolecular biology of the cell2013
The actin-microtubule cross-linking activity of Drosophila Short stop is regulated by intramolecular inhibition.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Global developmental delay and focal seizures in individuals with de novo truncating MACF1 variants.Human genomics · 2026Article
- Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles.bioRxiv : the preprint server for biology · 2025Article
- Microtubule actin crosslinking factor 1, a brain tumor oncoprotein (Review).Molecular and clinical oncology · 2025Review
- The Shot CH1 domain recognises a distinct form of F-actin during Drosophila oocyte determination.Development (Cambridge, England) · 2024Article
- Role of microtubule actin crosslinking factor 1 (MACF1) in bipolar disorder pathophysiology and potential in lithium therapeutic mechanism.Translational psychiatry · 2023Review
- Article
- Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility.Research (Washington, D.C.) · 2023Article
- TheMolecular biology of the cell · 2022Article
- Gatekeeper function for Short stop at the ring canals of the Drosophila ovary.Current biology : CB · 2021Article
- The Golgi microtubules regulate single cell durotaxis.EMBO reports · 2021Article
- The +TIP Navigator-1 is an actin-microtubule crosslinker that regulates axonal growth cone motility.The Journal of cell biology · 2020Article
- Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction.The Journal of cell biology · 2020Article
- Cytolinker Gas2L1 regulates axon morphology through microtubule-modulated actin stabilization.EMBO reports · 2019Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Actin-microtubule crosstalk in cell biology.Nature reviews. Molecular cell biology · 2019Review
- Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker.The Journal of cell biology · 2018Article
- Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity.Molecular biology of the cell · 2017Article
- Spectraplakin family proteins - cytoskeletal crosslinkers with versatile roles.Journal of cell science · 2017Review
- Structure of the ACF7 EF-Hand-GAR Module and Delineation of Microtubule Binding Determinants.Structure (London, England : 1993) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Actin and microtubule dynamics must be precisely coordinated during cell migration, mitosis, and morphogenesis--much of this coordination is mediated by proteins that physically bridge the two cytoskeletal networks. We have investigated the regulation of the Drosophila actin-microtubule cross-linker Short stop (Shot), a member of the spectraplakin family. Our data suggest that Shot's cytoskeletal cross-linking activity is regulated by an intramolecular inhibitory mechanism. In its inactive conformation, Shot adopts a "closed" conformation through interactions between its NH(2)-terminal actin-binding domain and COOH-terminal EF-hand-GAS2 domain. This inactive conformation is targeted to the growing microtubule plus end by EB1. On activation, Shot binds along the microtubule through its COOH-terminal GAS2 domain and binds to actin with its NH(2)-terminal tandem CH domains. We propose that this mechanism allows Shot to rapidly cross-link dynamic microtubules in response to localized activating signals at the cell cortex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.