ArticleJournal of virology2013
Antiviral RNA silencing initiated in the absence of RDE-4, a double-stranded RNA binding protein, in Caenorhabditis elegans.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.Journal of virology · 2026Article
- Viral RNA pUGylation promotes antiviral immunity inJournal of virology · 2025Article
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- A targeted genetic screen identifies Caenorhabditis elegans genes involved in RNAi-independent antiviral defense.Virology · 2025Article
- Review
- Natural variation in infection specificity of Caenorhabditis briggsae isolates by two RNA viruses.PLoS pathogens · 2024Article
- Small RNAs and chromatin in the multigenerational epigenetic landscape ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Transgene-Assisted Genetic Screen IdentifiesJournal of virology · 2018Article
- Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells.Oncology letters · 2018Article
- Caenorhabditis elegans as an Emerging Model for Virus-Host Interactions.Journal of virology · 2017Review
- Article
- Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes.Methods in molecular biology (Clifton, N.J.) · 2017Article
- The Shift of the Intestinal Microbiome in the Innate Immunity-Deficient MutantFrontiers in microbiology · 2017Article
- The evolving world of small RNAs from RNA viruses.Wiley interdisciplinary reviews. RNA · 2016Review
- Antiviral RNA Interference against Orsay Virus Is neither Systemic nor Transgenerational in Caenorhabditis elegans.Journal of virology · 2015Article
- Sensing viral RNAs by Dicer/RIG-I like ATPases across species.Current opinion in immunology · 2015Review
- Dicer's role as an antiviral: still an enigma.Current opinion in immunology · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Small interfering RNAs (siRNAs) processed from double-stranded RNA (dsRNA) of virus origins mediate potent antiviral defense through a process referred to as RNA interference (RNAi) or RNA silencing in diverse organisms. In the simple invertebrate Caenorhabditis elegans, the RNAi process is initiated by a single Dicer, which partners with the dsRNA binding protein RDE-4 to process dsRNA into viral siRNAs (viRNAs). Notably, in C. elegans this RNA-directed viral immunity (RDVI) also requires a number of worm-specific genes for its full antiviral potential. One such gene is rsd-2 (RNAi spreading defective 2), which was implicated in RDVI in our previous studies. In the current study, we first established an antiviral role by showing that rsd-2 null mutants permitted higher levels of viral RNA accumulation, and that this enhanced viral susceptibility was reversed by ectopic expression of RSD-2. We then examined the relationship of rsd-2 with other known components of RNAi pathways and established that rsd-2 functions in a novel pathway that is independent of rde-4 but likely requires the RNA-dependent RNA polymerase RRF-1, suggesting a critical role for RSD-2 in secondary viRNA biogenesis, likely through coordinated action with RRF-1. Together, these results suggest that RDVI in the single-Dicer organism C. elegans depends on the collective actions of both RDE-4-dependent and RDE-4-independent mechanisms to produce RNAi-inducing viRNAs. Our study reveals, for the first time, a novel siRNA-producing mechanism in C. elegans that bypasses the need for a dsRNA-binding protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.