ArticleAmerican journal of physiology. Cell physiology2013
Differential gene expression by endothelial cells under positive and negative streamwise gradients of high wall shear stress.
Article in American journal of physiology. Cell physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 4 syntheses or guidelines pooled it, 63 citations in OpenAlex.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- A Review of Biomechanics Analysis of the Umbilical-Placenta System With Regards to Diseases.Frontiers in physiology · 2021Pooled it
- What does computational fluid dynamics tell us about intracranial aneurysms? A meta-analysis and critical review.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Pooled it
- Association of wall shear stress with intracranial aneurysm rupture: systematic review and meta-analysis.Scientific reports · 2017Pooled it
- Possible hemodynamic mechanisms in the size change of cerebral aneurysms: an exploratory study.Quantitative imaging in medicine and surgery · 2026Article
- Biology, Physics, and Genetics of Intracranial Aneurysm Formation: A Review.Journal of neurological surgery. Part A, Central European neurosurgery · 2026Review
- Animal Models of Intracranial Aneurysms: History, Advances, and Future Perspectives.Translational stroke research · 2025Review
- The interdependent hemodynamic influence between abdominal aortic aneurysm and renal artery stenosis.Scientific reports · 2024Article
- Endothelial Cell Transcription Modulation in Cerebral Aneurysms After Endovascular Flow Diversion.Annals of biomedical engineering · 2024Article
- Endothelial Shear Stress Metrics Associate With Proinflammatory Pathways at the Culprit Site of Coronary Erosion.JACC. Basic to translational science · 2024Article
- Fluid-Dynamic Modeling of Flow in Embryonic Tissue Indicates That Lymphatic Valve Location Is Not Consistently Determined by the Local Fluid Shear or Its Gradient.Microcirculation (New York, N.Y. : 1994) · 2024Article
- A model with multiple intracranial aneurysms: possible hemodynamic mechanisms of aneurysmal initiation, rupture and recurrence.Chinese neurosurgical journal · 2024Article
- Cancer cell response to extrinsic and intrinsic mechanical cue: opportunities for tumor apoptosis strategies.Regenerative biomaterials · 2024Review
- The role of endothelial shear stress, shear stress gradient, and plaque topography in plaque erosion.Atherosclerosis · 2023Article
- Endothelial Responses to Curvature-Induced Flow Patterns in Engineered Cerebral Aneurysms.Journal of biomechanical engineering · 2023Article
- Review
- Omics of endothelial cell dysfunction in sepsis.Vascular biology (Bristol, England) · 2022Review
- Different Hemodynamic Characteristics and Resulting in Different Risks of Rupture Between Wide-Neck and Narrow-Neck Aneurysms.Frontiers in neurology · 2022Article
- Association Between Aneurysmal Hemodynamics and Rupture Risk of Unruptured Intracranial Aneurysms.Frontiers in neurology · 2022Article
- Endogenous animal models of intracranial aneurysm development: a review.Neurosurgical review · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Flow impingement at arterial bifurcations causes high frictional force [or wall shear stress (WSS)], and flow acceleration and deceleration in the branches create positive and negative streamwise gradients in WSS (WSSG), respectively. Intracranial aneurysms tend to form in regions with high WSS and positive WSSG. However, little is known about the responses of endothelial cells (ECs) to either positive or negative WSSG under high WSS conditions. We used cDNA microarrays to profile gene expression in cultured ECs exposed to positive or negative WSSG for 24 h in a flow chamber where WSS varied between 3.5 and 28.4 Pa. Gene ontology and biological pathway analysis indicated that positive WSSG favored proliferation, apoptosis, and extracellular matrix processing while decreasing expression of proinflammatory genes. To determine if similar responses occur in vivo, we examined EC proliferation and expression of the matrix metalloproteinase ADAMTS1 under high WSS and WSSG created at the basilar terminus of rabbits after bilateral carotid ligation. Precise hemodynamic conditions were determined by computational fluid dynamic simulations from three-dimensional angiography and mapped on immunofluorescence staining for the proliferation marker Ki-67 and ADAMTS1. Both proliferation and ADAMTS1 were significantly higher in ECs under positive WSSG than in adjacent regions of negative WSSG. Our results indicate that WSSG elicits distinct EC gene expression profiles and particular biological pathways including increased cell proliferation and matrix processing. Such EC responses may be important in understanding the mechanisms of intracranial aneurysm initiation at regions of high WSS and positive WSSG.
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