ArticleJournal of clinical microbiology2013
Discrimination of enterohemorrhagic Escherichia coli (EHEC) from non-EHEC strains based on detection of various combinations of type III effector genes.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 75 citations in OpenAlex.
- Developing a simple, cost-effective proof-of-concept vaccine candidate against EHEC for cattle.PloS one · 2026Article
- Emerging hybrid shigatoxigenic and enteropathogenicClinical microbiology reviews · 2025Review
- Shiga Toxin-ProducingMicroorganisms · 2025Review
- Using PathogenicFoods (Basel, Switzerland) · 2025Article
- Enhancing detection of STEC in the meat industry: insights into virulence of priority STEC.Frontiers in microbiology · 2025Article
- Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71.BMC veterinary research · 2024Article
- Combination of whole genome sequencing and supervised machine learning provides unambiguous identification ofFrontiers in microbiology · 2023Article
- Phylogenetic relationship and virulence composition ofFrontiers in microbiology · 2023Article
- Genomic Profiling of Non-O157 Shiga ToxigenicPHAGE (New Rochelle, N.Y.) · 2022Article
- Article
- Evaluation of high molecular weight DNA extraction methods for long-read sequencing of Shiga toxin-producing Escherichia coli.PloS one · 2022Article
- Whole-genome characterization of hemolytic uremic syndrome-causing Shiga toxin-producingVirulence · 2021Article
- Comparative Pathogenomics ofInfection and immunity · 2021Article
- Role of Recent Therapeutic Applications and the Infection Strategies of Shiga Toxin-ProducingFrontiers in cellular and infection microbiology · 2021Review
- Meningitis Caused byAnimals : an open access journal from MDPI · 2020Article
- Can Pathogenic and Nonpathogenic Bacteria Be Distinguished by Sensory Protein Abundance?Applied and environmental microbiology · 2020Article
- Review
- Is Shiga Toxin-ProducingMicroorganisms · 2020Article
- Virulence Profiling and Molecular Typing of Shiga Toxin-ProducingMicroorganisms · 2020Article
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) strains comprise a subgroup of Shiga-toxin (Stx)-producing E. coli (STEC) and are characterized by a few serotypes. Among these, seven priority STEC serotypes (O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7) are most frequently implicated in severe clinical illness worldwide. Currently, standard methods using stx, eae, and O-serogroup-specific gene sequences for detecting the top 7 EHEC serotypes bear the disadvantage that these genes can be found in non-EHEC strains as well. Here, we explored the suitability of ureD, espV, espK, espN, Z2098, and espM1 genes and combinations thereof as candidates for a more targeted EHEC screening assay. For a very large panel of E. coli strains (n = 1,100), which comprised EHEC (n = 340), enteropathogenic E. coli (EPEC) (n = 392), STEC (n = 193), and apathogenic strains (n = 175), we showed that these genetic markers were more prevalent in EHEC (67.1% to 92.4%) than in EPEC (13.3% to 45.2%), STEC (0.5% to 3.6%), and apathogenic E. coli strains (0 to 2.9%). It is noteworthy that 38.5% of the EPEC strains that tested positive for at least one of these genetic markers belonged to the top 7 EHEC serotypes, suggesting that such isolates might be Stx-negative derivatives of EHEC. The associations of espK with either espV, ureD, or Z2098 were the best combinations for more specific and sensitive detection of the top 7 EHEC strains, allowing detection of 99.3% to 100% of these strains. In addition, detection of 93.7% of the EHEC strains belonging to other serotypes than the top 7 offers a possibility for identifying new emerging EHEC strains.
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