ArticleJournal of clinical microbiology2013
Rapid visual detection of highly pathogenic Streptococcus suis serotype 2 isolates by use of loop-mediated isothermal amplification.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Validation and application of a visual LAMP assay for Mpox diagnosis - Insights from clinical samples in Thailand.MethodsX · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Development and application of a dual LAMP-LFD assay for the simultaneous detection ofFrontiers in cellular and infection microbiology · 2025Article
- Development of a Triplex qPCR Assay Based on the TaqMan Probe for the Detection ofMicroorganisms · 2024Article
- Integrating loop-mediated isothermal amplification with lateral flow assay to achieve a highly sensitive method for detectingHeliyon · 2024Article
- Contamination ofFoods (Basel, Switzerland) · 2024Article
- Rapid Diagnostic ofMicroorganisms · 2023Article
- Standardization of loop-mediated isothermal amplification for detection of D. nodosus and F. necrophorum causing footrot in sheep and goats.Tropical animal health and production · 2022Article
- Identification of a Novel Linear B Cell Epitope on the Sao Protein ofFrontiers in immunology · 2020Article
- A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection ofFrontiers in microbiology · 2019Article
- Contamination ofVeterinary world · 2019Article
- Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7.Journal of visualized experiments : JoVE · 2017Article
- Current Taxonomical Situation of Streptococcus suis.Pathogens (Basel, Switzerland) · 2016Review
- Development of a loop-mediated isothermal amplification assay for rapid detection of Trichosporon asahii in experimental and clinical samples.BioMed research international · 2015Article
- Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics.Expert review of molecular diagnostics · 2014Review
- Rapid detection of Streptococcus pneumoniae by real-time fluorescence loop-mediated isothermal amplification.Journal of thoracic disease · 2014Article
- Catabolite control protein A is an important regulator of metabolism inBiomedical reports · 2014Article
- Streptococcus suis infection: an emerging/reemerging challenge of bacterial infectious diseases?Virulence · 2014Review
- New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination.MethodsX · 2014Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that causes considerable economic losses to the pig industry and significantly threatens public health worldwide. The highly pathogenic S. suis 2, which contains the 89K pathogenicity island (PAI), has caused large-scale outbreaks of infections in humans, resulting in high mortality rates. In this study, we established two loop-mediated isothermal amplification (LAMP)-based assays that can rapidly detect S. suis 2 and the 89K PAI and can be performed simultaneously under the same conditions. Further, based on the findings of these two LAMP assays and using the same set of serially diluted DNA samples, we compared the sensitivities of different LAMP product detection methods, including SYBR green detection, gel electrophoresis, turbidimetry, calcein assays, and hydroxynaphthol blue detection. The results suggest that target genes can be amplified and detected within 48 min under 63°C isothermal conditions. The sensitivity of tests for S. suis 2 detection varies between detection methods and reaction systems, indicating that for each LAMP reaction system, multiple detection methods should be performed to select the optimal one. The sensitivities of the optimized methods (7.16 copies/reaction) in the present study were identical to those of the real-time PCR assay, and the test results for reference strains and clinical samples showed that these LAMP systems have high specificities. Thus, since the LAMP systems established in this study are simple, fast, and sensitive, they may have good clinical potential for detecting the highly pathogenic S. suis 2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.