ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 37 citations in OpenAlex.
- Orb2 RNA-binding activity promotes neural stem cell development and brain growth in Drosophila larvae.Cell reports · 2026Article
- The CPEB ortholog Orb2 regulates brain size through the TRIM-NHL RNA-binding protein, Brain tumor.bioRxiv : the preprint server for biology · 2025Article
- The Effects of the Association Between a High-Fat Diet and Physical Exercise on BDNF Expression in the Hippocampus: A Comprehensive Review.Life (Basel, Switzerland) · 2025Review
- Drosophila Atlastin regulates synaptic vesicle mobilization independent of bone morphogenetic protein signaling.Biological research · 2023Article
- Local BMP signaling: A sensor for synaptic activity that balances synapse growth and function.Current topics in developmental biology · 2022Review
- Neurexin and Neuroligins Maintain the Balance of Ghost and Satellite Boutons at theFrontiers in neuroanatomy · 2020Article
- A Novel Mutation in Brain Tumor Causes Both Neural Over-Proliferation and Neurodegeneration in AdultG3 (Bethesda, Md.) · 2018Article
- Review
- BMP signaling downstream of the Highwire E3 ligase sensitizes nociceptors.PLoS genetics · 2018Article
- Shep regulatesDevelopment (Cambridge, England) · 2018Article
- Neuroligin 4 regulates synaptic growth via the bone morphogenetic protein (BMP) signaling pathway at theThe Journal of biological chemistry · 2017Article
- Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins.RNA biology · 2017Article
- MAN1 Restricts BMP Signaling During Synaptic Growth in Drosophila.Cellular and molecular neurobiology · 2017Article
- Propofol Mitigates Learning and Memory Impairment After Electroconvulsive Shock in Depressed Rats by Inhibiting Autophagy in the Hippocampus.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Angelman Syndrome Protein Ube3a Regulates Synaptic Growth and Endocytosis by Inhibiting BMP Signaling in Drosophila.PLoS genetics · 2016Article
- Natural selection in a population of Drosophila melanogaster explained by changes in gene expression caused by sequence variation in core promoter regions.BMC evolutionary biology · 2016Article
- Coordinate post-transcriptional repression of Dpp-dependent transcription factors attenuates signal range during development.Development (Cambridge, England) · 2015Article
- The structural basis of Miranda-mediated Staufen localization during Drosophila neuroblast asymmetric division.Nature communications · 2015Article
- The translational regulator Cup controls NMJ presynaptic terminal morphology.Molecular and cellular neurosciences · 2015Article
- Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition.Genome biology · 2015Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precise regulation of synaptic growth is critical for the proper formation and plasticity of functional neural circuits. Identification and characterization of factors that regulate synaptic growth and function have been under intensive investigation. Here we report that brain tumor (brat), which was identified as a translational repressor in multiple biological processes, plays a crucial role at Drosophila neuromuscular junction (NMJ) synapses. Immunohistochemical analysis demonstrated that brat mutants exhibited synaptic overgrowth characterized by excess satellite boutons at NMJ terminals, whereas electron microscopy revealed increased synaptic vesicle size but reduced density at active zones compared with wild-types. Spontaneous miniature excitatory junctional potential amplitudes were larger and evoked quantal content was lower at brat mutant NMJs. In agreement with the morphological and physiological phenotypes, loss of Brat resulted in reduced FM1-43 uptake at the NMJ terminals, indicating that brat regulates synaptic endocytosis. Genetic analysis revealed that the actions of Brat at synapses are mediated through mothers against decapentaplegic (Mad), the signal transduction effector of the bone morphogenetic protein (BMP) signaling pathway. Furthermore, biochemical analyses showed upregulated levels of Mad protein but normal mRNA levels in the larval brains of brat mutants, suggesting that Brat suppresses Mad translation. Consistently, knockdown of brat by RNA interference in Drosophila S2 cells also increased Mad protein level. These results together reveal an important and previously unidentified role for Brat in synaptic development and endocytosis mediated by suppression of BMP signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.