ArticleStem cells translational medicine2013
Trichostatin A enhances differentiation of human induced pluripotent stem cells to cardiogenic cells for cardiac tissue engineering.
Article in Stem cells translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Microencapsulation-based cell therapies.Cellular and molecular life sciences : CMLS · 2022Review
- Culture Variabilities of Human iPSC-Derived Cerebral Organoids Are a Major Issue for the Modelling of Phenotypes Observed in Alzheimer's Disease.Stem cell reviews and reports · 2022Article
- Human iPSC-Cardiomyocytes as an Experimental Model to Study Epigenetic Modifiers of Electrophysiology.Cells · 2022Review
- Cardiac Differentiation of Mesenchymal Stem Cells: Impact of Biological and Chemical Inducers.Stem cell reviews and reports · 2021Review
- 4-phenylbutyrate exerts stage-specific effects on cardiac differentiation via HDAC inhibition.PloS one · 2021Article
- A Tissue Engineering Chamber for Continuous Pulsatile Electrical Stimulation of Vascularized Cardiac TissuesBioelectricity · 2020Article
- Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells.Stem cells international · 2019Article
- Bmi-1 high-expressing cells enrich cardiac stem/progenitor cells and respond to heart injury.Journal of cellular and molecular medicine · 2019Article
- Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.Cell death discovery · 2018Article
- The impact of in vitro cell culture duration on the maturation of human cardiomyocytes derived from induced pluripotent stem cells of myogenic origin.Cell transplantation · 2018Article
- Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor.Biomedical engineering online · 2017Article
- Article
- Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber.Journal of visualized experiments : JoVE · 2016Article
- Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications.PloS one · 2016Article
- Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells.Stem cells international · 2016Article
- Biomimetic 3D Tissue Models for Advanced High-Throughput Drug Screening.Journal of laboratory automation · 2015Review
- Bioengineering and Stem Cell Technology in the Treatment of Congenital Heart Disease.Journal of clinical medicine · 2015Review
- Cardiac disease modeling using induced pluripotent stem cell-derived human cardiomyocytes.World journal of stem cells · 2015Review
- SCL/TAL1-mediated transcriptional network enhances megakaryocytic specification of human embryonic stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Cardiac regeneration and diabetes.Regenerative medicine research · 2014Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human induced pluripotent stem (iPS) cells are a promising source of autologous cardiomyocytes to repair and regenerate myocardium for treatment of heart disease. In this study, we have identified a novel strategy to enhance cardiac differentiation of human iPS cells by treating embryoid bodies (EBs) with a histone deacetylase inhibitor, trichostatin A (TSA), together with activin A and bone morphogenetic protein 4 (BMP4). Over a narrow window of concentrations, TSA (1 ng/ml) directed the differentiation of human iPS cells into a cardiomyocyte lineage. TSA also exerted an additive effect with activin A (100 ng/ml) and BMP4 (20 ng/ml). The resulting cardiomyocytes expressed several cardiac-specific transcription factors and contractile proteins at both gene and protein levels. Functionally, the contractile EBs displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 μM) and carbachol (1 μM). Implanting microdissected beating areas of iPS cells into tissue engineering chambers in immunocompromised rats produced engineered constructs that supported their survival, and they maintained spontaneous contraction. Human cardiomyocytes were identified as compact patches of muscle tissue incorporated within a host fibrocellular stroma and were vascularized by host neovessels. In conclusion, human iPS cell-derived cardiomyocytes can be used to engineer functional cardiac muscle tissue for studying the pathophysiology of cardiac disease, for drug discovery test beds, and potentially for generation of cardiac grafts to surgically replace damaged myocardium.
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