ArticleGenes & development2013
Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.
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Who cites it
120 citing papers in PubMed, 233 citations in OpenAlex.
- Epigenetics in development, disease, and therapy: from mechanisms to clinical interventions.Science China. Life sciences · 2026Review
- Higher-order genome architecture of spermatids guides chromatin assembly in mature sperm.Research square · 2026Article
- The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness.Nature communications · 2026Article
- PRAMEL12 orchestrates spermiogenesis to ensure male fertility in mice.The Journal of biological chemistry · 2026Article
- ASB9 promotes ubiquitin-mediated degradation of TNP2 to facilitate histone-to-protamine transition in humans and mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- New Histone H4 variant and H2B variant Exhibits distinct genomic distributions, chromatin affinities, and dynamics throughout life and cell cycle of Trypanosoma cruzi.PLoS pathogens · 2026Article
- Article
- Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.Advances in experimental medicine and biology · 2026Review
- Dynamic architecture of mammalian paternal chromatin: histone-to-protamine exchange and post-fertilization reprogramming.Epigenetics & chromatin · 2025Review
- Evaluation of circANKLE2 & circL3MBTL4 -RNAs Expression in Fertile and Infertile Men.Biochemical genetics · 2025Article
- Epigenetic regulation by DNA methylation, histone modifications and chromatin remodeling complexes in controlling spermatogenesis and their dysfunction with male infertility.Cellular and molecular life sciences : CMLS · 2025Review
- H2B.W2, a spermatocyte-specific histone variant, disrupts nucleosome stability, and reduces chromatin compaction.Nucleic acids research · 2025Article
- Molecular Remodeling of the Sperm Proteome Following Varicocele Sclero-Embolization: Implications for Semen Quality Improvement.Proteomes · 2025Article
- The ABCs of the H2Bs: The histone H2B sequences, variants, and modifications.Trends in genetics : TIG · 2025Review
- Towards a kingdom of reproductive life - the core sperm proteome.Reproduction (Cambridge, England) · 2025Article
- Canonical and Variant Nucleosome Reprogramming from Sperm to Blastula.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Testis-specific H2B.W1 disrupts nucleosome integrity by reducing DNA-histone interactions.Nucleic acids research · 2024Article
- Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice.Scientific reports · 2024Article
- Roles of Histone H2B, H3 and H4 Variants in Cancer Development and Prognosis.International journal of molecular sciences · 2024Review
- Protein lysine crotonylation in cellular processions and disease associations.Genes & diseases · 2024Review
60 more citing papers are in PubMed but not listed here.
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Authors and funding
23 authors at 11 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.