ArticleBiotechnology for biofuels2013
Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystis sp. PCC 6803.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 76 citations in OpenAlex.
- Expression of tardigrade disordered proteins impacts the tolerance to biofuels in a model cyanobacteriumFrontiers in microbiology · 2022Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Article
- Recent advances in synthetic biology of cyanobacteria for improved chemicals production.Bioengineered · 2020Review
- Responses ofBiotechnology for biofuels · 2019Article
- Progress and perspectives on improving butanol tolerance.World journal of microbiology & biotechnology · 2017Review
- Metabolic responses to ethanol and butanol inBiotechnology for biofuels · 2017Article
- A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance ofBiotechnology for biofuels · 2017Article
- Systematic and functional identification of small non-coding RNAs associated with exogenous biofuel stress in cyanobacteriumBiotechnology for biofuels · 2017Article
- Towards elucidation of the toxic mechanism of copper on the model green alga Chlamydomonas reinhardtii.Ecotoxicology (London, England) · 2016Article
- Engineering cyanobacteria as photosynthetic feedstock factories.Photosynthesis research · 2015Review
- A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803.Molecular & cellular proteomics : MCP · 2014Article
- Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803.Microbial cell factories · 2014Article
- Metabolic design for cyanobacterial chemical synthesis.Photosynthesis research · 2014Review
- Genetic resources for advanced biofuel production described with the Gene Ontology.Frontiers in microbiology · 2014Review
- Protein Network Signatures Associated with Exogenous Biofuels Treatments in Cyanobacterium Synechocystis sp. PCC 6803.Frontiers in bioengineering and biotechnology · 2014Article
- Genetic instability in cyanobacteria - an elephant in the room?Frontiers in bioengineering and biotechnology · 2014Review
- Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803.Biotechnology for biofuels · 2014Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhotosynthetic cyanobacteria have been recently proposed as a 'microbial factory' to produce butanol due to their capability to utilize solar energy and CO2 as the sole energy and carbon sources, respectively. However, to improve the productivity, one key issue needed to be addressed is the low tolerance of the photosynthetic hosts to butanol.
resultsIn this study, we first applied a quantitative transcriptomics approach with a next-generation RNA sequencing technology to identify gene targets relevant to butanol tolerance in a model cyanobacterium Synechocystis sp. PCC 6803. The results showed that 278 genes were induced by the butanol exposure at all three sampling points through the growth time course. Genes encoding heat-shock proteins, oxidative stress related proteins, transporters and proteins involved in common stress responses, were induced by butanol exposure. We then applied GC-MS based metabolomics analysis to determine the metabolic changes associated with the butanol exposure. The results showed that 46 out of 73 chemically classified metabolites were differentially regulated by butanol treatment. Notably, 3-phosphoglycerate, glycine, serine and urea related to general stress responses were elevated in butanol-treated cells. To validate the potential targets, we constructed gene knockout mutants for three selected gene targets. The comparative phenotypic analysis confirmed that these genes were involved in the butanol tolerance.
conclusionThe integrated OMICS analysis provided a comprehensive view of the complicated molecular mechanisms employed by Synechocystis sp. PCC 6803 against butanol stress, and allowed identification of a series of potential gene candidates for tolerance engineering in cyanobacterium Synechocystis sp. PCC 6803.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.