ArticleBMC cancer2013
Genetic and epigenetic analysis of putative breast cancer stem cell models.
Article in BMC cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Computational development of mushroom-6-glucan/paclitaxel as a synergistic complementary medicine for breast cancer therapy.BMC complementary medicine and therapies · 2025Article
- A Stemness and EMT Based Gene Expression Signature Identifies Phenotypic Plasticity and is A Predictive but Not Prognostic Biomarker for Breast Cancer.Journal of Cancer · 2020Article
- Chronic Oxidative Stress Promotes Molecular Changes Associated with Epithelial Mesenchymal Transition, NRF2, and Breast Cancer Stem Cell Phenotype.Antioxidants (Basel, Switzerland) · 2019Article
- Interrogation of Phenotypic Plasticity between Epithelial and Mesenchymal States in Breast Cancer.Journal of clinical medicine · 2019Article
- The Evolving Landscape of Cancer Stem Cells and Ways to Overcome Cancer Heterogeneity.Cancers · 2019Review
- Polo-like kinase 1 (Plk1) inhibition synergizes with taxanes in triple negative breast cancer.PloS one · 2019Article
- Article
- Primary patient-derived lung adenocarcinoma cell culture challenges the association of cancer stem cells with epithelial-to-mesenchymal transition.Scientific reports · 2017Article
- GLI1-mediated regulation of side population is responsible for drug resistance in gastric cancer.Oncotarget · 2017Article
- MicroRNA-17 is downregulated in esophageal adenocarcinoma cancer stem-like cells and promotes a radioresistant phenotype.Oncotarget · 2017Article
- Cancer cells exhibit clonal diversity in phenotypic plasticity.Open biology · 2017Article
- Genetic profiling of putative breast cancer stem cells from malignant pleural effusions.PloS one · 2017Article
- Role of epigenetic modifications in luminal breast cancer.Epigenomics · 2015Review
- Downregulation of E-cadherin expression in breast cancer by promoter hypermethylation and its relation with progression and prognosis of tumor.Medical oncology (Northwood, London, England) · 2014Article
- Sequencing of breast cancer stem cell populations indicates a dynamic conversion between differentiation states in vivo.Breast cancer research : BCR · 2014Article
- Association of microRNAs and pathologic response to preoperative chemotherapy in triple negative breast cancer: preliminary report.Molecular biology reports · 2014Article
- Genome-Wide DNA Methylation Pattern of Cancer Stem Cells in Esophageal Cancer.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCancer stem cell model hypothesizes existence of a small proportion of tumor cells capable of sustaining tumor formation, self-renewal and differentiation. In breast cancer, these cells were found to be associated with CD44⁺CD24-low and ALDH⁺ phenotype. Our study was performed to evaluate the suitability of current approaches for breast cancer stem cell analyses to evaluate heterogeneity of breast cancer cells through their extensive genetic and epigenetic characterization.
methodsBreast cancer cell lines MCF7 and SUM159 were cultured in adherent conditions and as mammospheres. Flow cytometry sorting for CD44, CD24 and ALDH was performed. Sorted and unsorted populations, mammospheres and adherent cell cultures were subjected to DNA profiling by array CGH and methylation profiling by Epitect Methyl qPCR array. Methylation status of selected genes was further evaluated by pyrosequencing. Functional impact of methylation was evaluated by mRNA analysis for selected genes.
resultsArray CGH did not reveal any genomic differences. In contrast, putative breast cancer stem cells showed altered methylation levels of several genes compared to parental tumor cells.
conclusionsOur results underpin the hypothesis that epigenetic mechanisms seem to play a major role in the regulation of CSCs. However, it is also clear that more efficient methods for CSC enrichment are needed. This work underscores requirement of additional approaches to reveal heterogeneity within breast cancer.
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