ArticleBiomicrofluidics2012
On-chip three-dimensional tumor spheroid formation and pump-less perfusion culture using gravity-driven cell aggregation and balanced droplet dispensing.
Article in Biomicrofluidics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Lung carcinoma spheroids embedded in a microfluidic platform.Cytotechnology · 2021Article
- Fluorinated Chitosan Microgels to Overcome Internal Oxygen Transport Deficiencies in Microtissue Culture Systems.Advanced biosystems · 2020Article
- Microfluidic technologies to engineer mesenchymal stem cell aggregates-applications and benefits.Biophysical reviews · 2020Review
- A Dynamic 3D Tumor Spheroid Chip Enables More Accurate Nanomedicine Uptake Evaluation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Probing tumor microtissue formation and epithelial-mesenchymal transition on a well-mesh microchip.Biomicrofluidics · 2019Article
- Microfluidic Based Optical Microscopes on Chip.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Review
- 3D modeling of cancer stem cell niche.Oncotarget · 2018Review
- Electrical Impedance Spectroscopy for Microtissue Spheroid Analysis in Hanging-Drop Networks.ACS sensors · 2016Article
- Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform.Lab on a chip · 2016Article
- Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform.Lab on a chip · 2016Article
- Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-Cancer Drug Testing in 3D Culture.Cellular and molecular bioengineering · 2014Article
- Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement.Biomicrofluidics · 2014Article
- Accurate dispensing system for single oocytes using air ejection.Biomicrofluidics · 2013Article
- Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy.Biomicrofluidics · 2013Article
- Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?Biomicrofluidics · 2013Article
- Bacterial aggregation and biofilm formation in a vortical flow.Biomicrofluidics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper presents a spheroid chip in which three-dimensional (3D) tumor spheroids are not only formed by gravity-driven cell aggregation but also cultured at the perfusion rates controlled by balanced droplet dispensing without fluidic pumps. The previous spheroid chips require additional off-chip processes of spheroid formation and extraction as well as bulky components of fluidic pumps. However, the present spheroid chip, where autonomous medium droplet dispensers are integrated on a well array, achieves the on-chip 3D tumor spheroid formation and perfusion culture using simple structure without bulky fluidic pumps. In the experimental study, we demonstrated that the spheroid chip successfully forms 3D tumor spheroids in the wide diameter range of 220 μm-3.2 mm (uniformity > 90%) using H358, H23, and A549 non-small cell lung cancer cells. At the pump-less perfusion culture (Q = 0.1-0.3 μl/min) of spheroids, the number of H358 cells in the spheroid increased up to 50% from the static culture (Q = 0 μl/min) and the viability of the cultured cells also increased about 10%. Therefore, we experimentally verified that the perfusion environment created by the spheroid chip offers a favourable condition to the spheroids with high increase rate and viability. The present chip achieves on-chip 3D tumor spheroid formation and pump-less perfusion culture with simple structure, thereby exhibiting potential for use in integrated in-vivo-like cell culture systems.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.