ArticleMolecular & cellular proteomics : MCP2013
Amine-reactive neutron-encoded labels for highly plexed proteomic quantitation.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- High-Throughput Metabolomics using 96-plex Isotope Tagging.Analytical chemistry · 2024Article
- Strategies for Increasing the Depth and Throughput of Protein Analysis by plexDIA.Journal of proteome research · 2023Review
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Chemical isotope labeling for quantitative proteomics.Mass spectrometry reviews · 2023Review
- Neutron encoded derivatization of endothelial cell lysates for quantitation of aldehyde metabolites using nESI-LC-HRMS.Analytica chimica acta · 2022Article
- 21-plex DiLeu Isobaric Tags for High-Throughput Quantitative Proteomics.Analytical chemistry · 2020Article
- An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis.Molecular cell · 2019Article
- Relative Quantitation of Neuropeptides at Multiple Developmental Stages of the American Lobster Using N, N-Dimethyl Leucine Isobaric Tandem Mass Tags.ACS chemical neuroscience · 2018Article
- Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its Orchestration of Mitochondrial Biogenesis.Cell systems · 2018Article
- Multi-omic Mitoprotease Profiling Defines a Role for Oct1p in Coenzyme Q Production.Molecular cell · 2017Article
- A Split-Abl Kinase for Direct Activation in Cells.Cell chemical biology · 2017Article
- Mass Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags for Multiplex Quantitative Proteomics.Analytical chemistry · 2017Article
- Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells.Analytical chemistry · 2017Article
- Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling.Nature biotechnology · 2016Article
- Biomarker discovery in mass spectrometry-based urinary proteomics.Proteomics. Clinical applications · 2016Review
- Neucode Labels for Multiplexed, Absolute Protein Quantification.Analytical chemistry · 2016Article
- Phosphoproteomics in the Age of Rapid and Deep Proteome Profiling.Analytical chemistry · 2016Review
- NeuCode Labeling in Nematodes: Proteomic and Phosphoproteomic Impact of Ascaroside Treatment in Caenorhabditis elegans.Molecular & cellular proteomics : MCP · 2015Article
- Comprehensive Temporal Protein Dynamics during the Diauxic Shift in Saccharomyces cerevisiae.Molecular & cellular proteomics : MCP · 2015Article
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Authors and funding
8 authors.
Funding
Abstract
We describe a novel amine-reactive chemical label that exploits differential neutron-binding energy between (13)C and (15)N isotopes. These neutron-encoded (NeuCode) chemical labels enable up to 12-plex MS1-based protein quantification. Each structurally identical, but isotopically unique, tag is encoded with a 12.6-mDa mass difference-relative to its nearest neighbor-so that peptides bearing these NeuCode signatures do not increase spectral complexity and are detected only upon analysis with very high mass-resolving powers. We demonstrate that the method provides quantitative performance that is comparable to both metabolic labeling and isobaric tagging while combining the benefits of both strategies. Finally, we employ the tags to characterize the proteome of Saccharomyces cerevisiae during the diauxic shift, a metabolic transition from fermentation to aerobic respiration.
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