ArticleMolecular & cellular proteomics : MCP2013
Molecular responses of mouse macrophages to copper and copper oxide nanoparticles inferred from proteomic analyses.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages.International journal of molecular sciences · 2020Pooled it
- Copper oxide nanoparticles: from EU-regulatory landscape and mechanistic toxicity towards a cuproptosis-linked adverse outcome pathway.Archives of toxicology · 2026Review
- Metal Oxide Doping Modulates the Performances of Copper Oxide Nanoparticular Biocides.Nanomaterials (Basel, Switzerland) · 2026Article
- Multifunctional Cu-DEOD porous polymeric nanocomposite for broad-spectrum biomedical applications.RSC advances · 2025Article
- Identification of copper metabolism-related subtypes, the development of a prognosis model, and characterization of the immune landscape in colorectal cancer.Discover oncology · 2025Article
- The VVBlue assay: a plate-readable, dye exclusion-based cell viability assay for the toxicological testing of chemicals.RSC advances · 2025Article
- The role of depression in the progression of tumor cell inflammation and the potential regulatory mechanisms of aerobic exercise: a narrative review from molecular and cellular perspectives.Frontiers in psychiatry · 2025Review
- Recent Trends in Biomedical Applications of CuInternational journal of nanomedicine · 2025Review
- Interplay of Oxidative Stress, Inflammation, and Autophagy in RAW 264.7 Murine Macrophage Cell Line Challenged with Si/SiOMaterials (Basel, Switzerland) · 2023Article
- Novel Anti-Neuroinflammatory Properties of a Thiosemicarbazone-Pyridylhydrazone Copper(II) Complex.International journal of molecular sciences · 2022Article
- Nanoparticle Effects on Stress Response Pathways and Nanoparticle-Protein Interactions.International journal of molecular sciences · 2022Review
- Copper oxide nanoparticles trigger macrophage cell death with misfolding of Cu/Zn superoxide dismutase 1 (SOD1).Particle and fibre toxicology · 2022Article
- Immediate and Sustained Effects of Cobalt and Zinc-Containing Pigments on Macrophages.Frontiers in immunology · 2022Article
- Genotoxicity of aluminium oxide, iron oxide, and copper nanoparticles in mouse bone marrow cells.Arhiv za higijenu rada i toksikologiju · 2021Article
- Influences of Nanoparticles Characteristics on the Cellular Responses: The Example of Iron Oxide and Macrophages.Nanomaterials (Basel, Switzerland) · 2020Article
- Metal Oxide Nanoparticles in Therapeutic Regulation of Macrophage Functions.Nanomaterials (Basel, Switzerland) · 2019Review
- Effects of Iron Oxide Nanoparticles (γ-FeInternational journal of molecular sciences · 2019Article
- Article
- Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials.Analytical and bioanalytical chemistry · 2018Review
- A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular responses of macrophages to copper-based nanoparticles have been investigated via a combination of proteomic and biochemical approaches, using the RAW264.7 cell line as a model. Both metallic copper and copper oxide nanoparticles have been tested, with copper ion and zirconium oxide nanoparticles used as controls. Proteomic analysis highlighted changes in proteins implicated in oxidative stress responses (superoxide dismutases and peroxiredoxins), glutathione biosynthesis, the actomyosin cytoskeleton, and mitochondrial proteins (especially oxidative phosphorylation complex subunits). Validation studies employing functional analyses showed that the increases in glutathione biosynthesis and in mitochondrial complexes observed in the proteomic screen were critical to cell survival upon stress with copper-based nanoparticles; pharmacological inhibition of these two pathways enhanced cell vulnerability to copper-based nanoparticles, but not to copper ions. Furthermore, functional analyses using primary macrophages derived from bone marrow showed a decrease in reduced glutathione levels, a decrease in the mitochondrial transmembrane potential, and inhibition of phagocytosis and of lipopolysaccharide-induced nitric oxide production. However, only a fraction of these effects could be obtained with copper ions. In conclusion, this study showed that macrophage functions are significantly altered by copper-based nanoparticles. Also highlighted are the cellular pathways modulated by cells for survival and the exemplified cross-toxicities that can occur between copper-based nanoparticles and pharmacological agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.