ArticleJournal of assisted reproduction and genetics2013
Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 64 citations in OpenAlex.
- Intrafollicular immature oocyte transfer (IFIOT): technical advances, biological insights and applications in reproductive biotechnologies.Animal reproduction · 2026Article
- Approaches toAnimal reproduction · 2025Article
- Molecular Mechanisms Determining Mammalian Oocyte Quality with the Treatment of Cancer Therapy.Advances in anatomy, embryology, and cell biology · 2024Review
- Mammalian cumulus-oocyte complex communication: a dialog through long and short distance messaging.Journal of assisted reproduction and genetics · 2022Review
- Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes.Veterinary medicine and science · 2021Article
- Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Involvement of GJA1 and Gap Junctional Intercellular Communication between Cumulus Cells and Oocytes from Women with PCOS.BioMed research international · 2020Article
- Intrafollicular barriers and cellular interactions during ovarian follicle development.Animal reproduction · 2019Article
- Pre-IVM treatment with C-type natriuretic peptide in the presence of cysteamine enhances bovine oocytes antioxidant defense ability and developmental competenceIranian journal of veterinary research · 2019Article
- Contributions from the ovarian follicular environment to oocyte function.Animal reproduction · 2018Article
- Transporting cumulus complexes using novel meiotic arresting conditions permits maintenance of oocyte developmental competence.Journal of assisted reproduction and genetics · 2017Article
- Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes.Scientific reports · 2017Article
- Levels of cyclic-AMP and cyclic-GMP in porcine oocyte-cumulus complexes and cumulus-free oocytes derived from small and middle follicles during the first 24-hour period of in vitro maturation.The Journal of reproduction and development · 2017Article
- PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis.Cell cycle (Georgetown, Tex.) · 2016Article
- The variable success of in vitro maturation: can we do better?Animal reproductionArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis study was aimed to test the hypothesis that gap junction mediated communications (GJC) are required to allow the progressive chromatin configuration remodeling (from GV1 to GV3) process to occur in fully grown oocytes in order to gain the final step of developmental competence acquisition, and that a premature disruption of GJC can alter this process.
methodsBovine cumulus-oocytes complexes collected from medium antral follicles were cultured for 2, 4, 6 and 8 h in the presence of 10(-4) IU/ml of r-hFSH and with 2 mM of the non-selective PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) to prevent meiotic resumption. GJC functionality and chromatin configuration were monitored during the culture period. After meiotic arrest, the developmental capability of oocytes was assessed after IVM and IVF.
resultsIBMX was effective in significantly sustaining GJC up to 6 h and maintaining meiotic arrest, when compared to control group. Moreover, the percentage of oocytes with less condensed chromatin (GV1) decreased within 4 h of culture, while the proportion of GV2 oocytes gradually increased up to 6 h. Interestingly, a decline in the proportion of GV2 oocytes and an increase in the proportion of GV3 oocytes were observed after 6 h of culture, when the major drop of GJC occurred. On the contrary, when GJC were uncoupled by adding 3 mM of 1-heptanol or through cumulus cells removal, chromatin condensation occurred rapidly throughout the culture period, more promptly in denuded oocytes. Moreover, the maintenance of GJC during meiotic arrest was accompanied by a significant increase of developmental competence compared to the control, as indicated by a higher percentage of hatched blastocysts and blastocyst cell number.
conclusionsAltogether, our data indicate that both paracrine and junctional mechanisms are involved in modulating large-scale chromatin structure during the final phase of oocyte differentiation.
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