ArticleInternational journal of environmental research and public health2013
Optimization of pulse-field gel electrophoresis for subtyping of Klebsiella pneumoniae.
Article in International journal of environmental research and public health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
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- Antimicrobial resistance, virulence genes profiles and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae strains from captive giant pandas (Ailuropoda melanoleuca).BMC veterinary research · 2024Article
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- Molecular epidemiology, risk factors, and outcomes of carbapenem-resistantFrontiers in microbiology · 2023Article
- Molecular typing methods & resistance mechanisms of MDRAIMS microbiology · 2023Review
- Antimicrobial Resistance Patterns and Clonal Distribution ofAntibiotics (Basel, Switzerland) · 2022Article
- Surveillance of Antimicrobial Resistance in Hospital Wastewater: Identification of Carbapenemase-ProducingAntibiotics (Basel, Switzerland) · 2022Article
- Polyclonal Endemicity of Carbapenemase-ProducingAntibiotics (Basel, Switzerland) · 2022Article
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- An outbreak of extensively drug-resistant and hypervirulentFrontiers in cellular and infection microbiology · 2022Article
- Molecular Characterization of Antibiotic Resistance and Genetic Diversity ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Biofilm Formation of ClinicalPathogens (Basel, Switzerland) · 2021Article
- First Report ofMicroorganisms · 2021Article
- Clonal Dissemination of KPC-2, VIM-1, OXA-48-ProducingPolish journal of microbiology · 2021Article
- Emergence ofInfection and drug resistance · 2021Article
- Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-ResistantFrontiers in microbiology · 2021Article
- Heterogeneity and Diversity ofAntimicrobial agents and chemotherapy · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pneumoniae. By software analysis and pilot study, AvrII was chosen as another PFGE enzyme. Both XbaI- and AvrII-PFGE gave D-values higher than 0.99 for 69 K. pneumoniae isolated from different sources. Our results also showed good typeability, reproducibility of both XbaI- and AvrII-PFGE for K. pneumoniae subtyping. Furthermore, the established PFGE method also had good discriminatory power to distinguish outbreak K. pneumoniae strains and a high degree of consistency with multilocus sequence typing method. A rapid PFGE protocol was established here, which could be used for genotyping and other researches of K. pneumoniae.
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