ArticleAmerican journal of physiology. Endocrinology and metabolism2013
Regulation of lipid synthesis genes and milk fat production in human mammary epithelial cells during secretory activation.
Article in American journal of physiology. Endocrinology and metabolism, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 88 citations in OpenAlex.
- De novo synthesis of milk triglycerides in humans.American journal of physiology. Endocrinology and metabolism · 2014Trial
- Insulin resistance is associated with mammary mitochondrial dysfunction at the onset of human lactation.The Journal of clinical investigation · 2026Article
- Methods for Preserving Human Milk Cellular and Milk Fat Globule RNA.Journal of mammary gland biology and neoplasia · 2026Article
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- Refractive index of milk fat globules and extracellular vesicles in human milk.Biophotonics discovery · 2026Article
- Molecular Response of Simmental Cows to Negative Energy Balance: Regulation of Interleukin-6 and Plasminogen During Early Lactation.International journal of molecular sciences · 2025Article
- Genomic characterization of normal and aberrant human milk production.Science advances · 2025Article
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- Review: genetic background of milk fatty acid synthesis in bovines.Tropical animal health and production · 2023Review
- Human Milk Lipids and Small Metabolites: Maternal and Microbial Origins.Metabolites · 2023Review
- Fur removal promotes an earlier expression of involution-related genes in mammary gland of lactating mice.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
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- MYCN-driven fatty acid uptake is a metabolic vulnerability in neuroblastoma.Nature communications · 2022Article
- Dietary Intake of Polyphenols Enhances Executive/Attentional Functioning and Memory with an Improvement of the Milk Lipid Profile of Postpartum Women from Argentina.Journal of Intelligence · 2022Article
- Elucidating Human Milk Oligosaccharide biosynthetic genes through network-based multi-omics integration.Nature communications · 2022Article
- Cellular and transcriptional diversity over the course of human lactation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Lactational Changes of Phospholipids Content and Composition in Chinese Breast Milk.Nutrients · 2022Article
- Mammary epithelial cell transcriptome reveals potential roles of lncRNAs in regulating milk synthesis pathways in Jersey and Kashmiri cattle.BMC genomics · 2022Article
- Effect of Delta-9-tetrahydrocannabinol and cannabidiol on milk proteins and lipid levels in HC11 cells.PloS one · 2022Article
- Depletion and enrichment of phytosterols in soybean oil lipid emulsions directly associate with serum markers of cholestasis in preterm parenteral nutrition-fed pigs.JPEN. Journal of parenteral and enteral nutrition · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Expression of genes for lipid biosynthetic enzymes during initiation of lactation in humans is unknown. Our goal was to study mRNA expression of lipid metabolic enzymes in human mammary epithelial cell (MEC) in conjunction with the measurement of milk fatty acid (FA) composition during secretory activation. Gene expression from mRNA isolated from milk fat globule (MFG) and milk FA composition were measured from 6 h to 42 days postpartum in seven normal women. Over the first 96 h postpartum, daily milk fat output increased severalfold and mirrored expression of genes for all aspects of lipid metabolism and milk FA production, including lipolysis at the MEC membrane, FA uptake from blood, intracellular FA transport, de novo FA synthesis, FA and glycerol activation, FA elongation, FA desaturation, triglyceride synthesis, cholesterol synthesis, and lipid droplet formation. Expression of the gene for a key lipid synthesis regulator, sterol regulatory element-binding transcription factor 1 (SREBF1), increased 2.0-fold by 36 h and remained elevated over the study duration. Expression of genes for estrogen receptor 1, thyroid hormone-responsive protein, and insulin-induced 2 increased progressively to plateau by 96 h. In contrast, mRNA of peroxisome proliferator-activated receptor-γ decreased severalfold. With onset of lactation, increased de novo synthesis of FA was the most prominent change in milk FA composition and mirrored the expression of FA synthesis genes. In conclusion, milk lipid synthesis and secretion in humans is a complex process requiring the orchestration of a wide variety of pathways of which SREBF1 may play a primary role.
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