ArticleMolecular and cellular biology2013
Cap completion and C-terminal repeat domain kinase recruitment underlie the initiation-elongation transition of RNA polymerase II.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Aberrant RNA polymerase initiation and processivity on the genome of a herpes simplex virus 1 mutant lacking ICP27.Journal of virology · 2024Article
- A Revision of Herpes Simplex Virus Type 1 Transcription: First, Repress; Then, Express.Microorganisms · 2024Review
- The Nuclear Cap-Binding Complex, a multitasking binding partner of RNA polymerase II transcripts.Journal of biochemistry · 2023Review
- Ceg1 depletion reveals mechanisms governing degradation of non-capped RNAs in Saccharomyces cerevisiae.Communications biology · 2023Article
- Review
- Conditional depletion of transcriptional kinases Ctk1 and Bur1 and effects on co-transcriptional spliceosome assembly and pre-mRNA splicing.RNA biology · 2021Article
- Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The nuclear cap-binding complex as choreographer of gene transcription and pre-mRNA processing.Genes & development · 2020Review
- Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.PLoS genetics · 2020Article
- Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction.eLife · 2020Article
- Interplay of mRNA capping and transcription machineries.Bioscience reports · 2020Review
- The capping enzyme facilitates promoter escape and assembly of a follow-on preinitiation complex for reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila.FEBS letters · 2019Article
- Pre-mRNA Splicing in the Nuclear Landscape.Cold Spring Harbor symposia on quantitative biology · 2019Article
- Single-molecule nascent RNA sequencing identifies regulatory domain architecture at promoters and enhancers.Nature genetics · 2018Article
- A Mechanism for the Activation of the Influenza Virus Transcriptase.Molecular cell · 2018Article
- The essential and multifunctional TFIIH complex.Protein science : a publication of the Protein Society · 2018Review
- Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage.Nature communications · 2018Article
- Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.Nature structural & molecular biology · 2017Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
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3 authors.
Funding
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Abstract
After transcription initiation, RNA polymerase (Pol) II escapes from the promoter and recruits elongation factors. The molecular basis for the initiation-elongation factor exchange during this transition remains poorly understood. Here, we used chromatin immunoprecipitation (ChIP) to elucidate the initiation-elongation transition of Pol II in the budding yeast Saccharomyces cerevisiae. We show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1. Genome-wide occupancy for Cet1 and Ceg1 is restricted to the transcription start site (TSS), whereas occupancy for Abd1 peaks at ~110 nucleotides downstream, and occupancy for the cap-binding complex (CBC) rises subsequently. Abd1 and CBC are important for recruitment of the kinases Ctk1 and Bur1, which promote elongation and capping enzyme release. These results suggest that cap completion stimulates productive Pol II elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.