ArticleProceedings of the National Academy of Sciences of the United States of America2013
Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Fluorogenic Coupled Assays Reveal Catalytic Properties, Inhibition Constants and Cellular Location of Mucin-Active Carbohydrate Sulfatases.Angewandte Chemie (International ed. in English) · 2026Article
- Shifting Mycobacterial Serine Hydrolase Activity Visualized Using Multi-Layer In-Gel Activity Assays.Molecules (Basel, Switzerland) · 2024Article
- Chemical approaches to unraveling the biology of mycobacteria.Cell chemical biology · 2023Review
- Two Color Imaging of Different Hypoxia Levels in Cancer Cells.Journal of the American Chemical Society · 2023Article
- Genome-Wide Study of Drug ResistantMicroorganisms · 2022Article
- The discovery of penta-peptides inhibiting the activity of the formylglycine-generating enzyme and their potential antibacterial effects againstRSC advances · 2022Article
- Feasibility of novel approaches to detect viableFrontiers in medicine · 2022Review
- Improved Conventional and New Approaches in the Diagnosis of Tuberculosis.Frontiers in microbiology · 2022Review
- Chemiluminescent Protease Probe for Rapid, Sensitive, and Inexpensive Detection of LiveACS central science · 2021Article
- Rapid and specific labeling of single liveScience translational medicine · 2018Article
- Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays.AMB Express · 2017Article
- Optical visualization and quantification of enzyme activity using dynamic droplet lenses.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Small-Molecule Probes Reveal Esterases with Persistent Activity in Dormant and Reactivating Mycobacterium tuberculosis.ACS infectious diseases · 2016Article
- Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates.ACS chemical biology · 2016Article
- Progress and prospects for small-molecule probes of bacterial imaging.Nature chemical biology · 2016Review
- Synthesis and Evaluation of Cytosolic Phospholipase A(2) Activatable Fluorophores for Cancer Imaging.Bioconjugate chemistry · 2015Article
- Far-red fluorogenic probes for esterase and lipase detection.Chembiochem : a European journal of chemical biology · 2015Article
- Fluorogenic probes with substitutions at the 2 and 7 positions of cephalosporin are highly BlaC-specific for rapid Mycobacterium tuberculosis detection.Angewandte Chemie (International ed. in English) · 2014Article
- An expanded set of fluorogenic sulfatase activity probes.Chembiochem : a European journal of chemical biology · 2014Article
- Bright building blocks for chemical biology.ACS chemical biology · 2014Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.