ArticleMarine biotechnology (New York, N.Y.)2014
Identification and characterization of microRNAs in pearl oyster Pinctada martensii by Solexa deep sequencing.
Article in Marine biotechnology (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- A microRNA Catalogue for Red Abalone Larvae Reveals Developmental and Acidification-Associated Abundance Patterns.Marine biotechnology (New York, N.Y.) · 2026Article
- SmithHunter: a workflow for the identification of candidate smithRNAs and their targets.BMC bioinformatics · 2024Article
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- miR-10a-3p Participates in Nacre Formation in the Pearl Oyster Pinctada fucata martensii by Targeting NPY.Marine biotechnology (New York, N.Y.) · 2023Article
- Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2022Article
- MiRNA-mRNA Integration Analysis Reveals the Regulatory Roles of MiRNAs in Shell Pigmentation of the Manila clam (Ruditapes philippinarum).Marine biotechnology (New York, N.Y.) · 2021Article
- Discovery and functional understanding of MiRNAs in molluscs: a genome-wide profiling approach.RNA biology · 2021Article
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- Digging into bivalve miRNAomes: between conservation and innovation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
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- Integrated analysis of microRNA and mRNA expression profiles in Crassostrea gigas to reveal functional miRNA and miRNA-targets regulating shell pigmentation.Scientific reports · 2020Article
- Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1.BMC genomics · 2020Article
- A species-specific miRNA participates in biomineralization by targeting CDS regions of Prisilkin-39 and ACCBP in Pinctada fucata.Scientific reports · 2020Article
- Identification and Characterization of microRNAs and Their Predicted Functions in Biomineralization in the Pearl Oyster (Biology · 2019Article
- PfmPif97-like regulated by Pfm-miR-9b-5p participates in shell formation in Pinctada fucata martensii.PloS one · 2019Article
- Identification and characteristics of muscle growth-related microRNA in the Pacific abalone, Haliotis discus hannai.BMC genomics · 2018Article
- SmithRNAs: Could Mitochondria "Bend" Nuclear Regulation?Molecular biology and evolution · 2017Article
- Article
- Long Non-Coding RNAs (lncRNAs) of Sea Cucumber: Large-Scale Prediction, Expression Profiling, Non-Coding Network Construction, and lncRNA-microRNA-Gene Interaction Analysis of lncRNAs in Apostichopus japonicus and Holothuria glaberrima During LPS Challenge and Radial Organ Complex Regeneration.Marine biotechnology (New York, N.Y.) · 2016Article
- Computational prediction of candidate miRNAs and their potential functions in biomineralization in pearl oyster Pinctada martensii.Saudi journal of biological sciences · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are short-nucleotide RNA molecules that function as negative regulators of gene expression in various organisms. However, miRNAs of Pinctada martensii have not been reported yet. P. martensii is one of the main species cultured for marine pearl production in China and Japan. In order to obtain the repertoire of miRNAs in P. martensii, we constructed and sequenced small RNA libraries prepared from P. martensii by Solexa deep sequencing technology and got a total of 27,479,838 reads representing 3,176,630 distinct sequences. After removing tRNAs, rRNAs, snRNAs, and snoRNAs, 10,596,306 miRNA reads representing 18,050 distinct miRNA reads were obtained. Based on sequence similarity and hairpin structure prediction, 258 P. martensii miRNAs (pm-miRNA) were identified. Among these pm-miRNAs, 205 were conserved across the species, whereas 53 were specific for P. martensii. The 3' end sequence of U6 snRNA was obtained from P. martensii by 3' rapid amplification of cDNA end PCR reaction and sequence-directed cloning. Eight conserved pm-miRNAs and two novel pm-miRNAs were validated by stem-loop quantitative real-time PCR with U6 snRNA as an internal reference gene. pm-miRNAs and the reported biomineralization-related genes were subjected to target analysis by using target prediction tools. Some of the pm-miRNAs, such as miR-2305 and miR-0046, were predicted to participate in biomineralization by regulating the biomineralization-related genes. Thus, this study demonstrated a large-scale characterization of pm-miRNAs and their potential function in biomineralization, providing a foundation to understand shell formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.