ArticleMarine biotechnology (New York, N.Y.)2013
DNA methylation is crucial for the early development in the Oyster C. gigas.
Article in Marine biotechnology (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 146 citations in OpenAlex.
- Molecular Understanding of Cryopreservation of Marine Invertebrates: Achievements and New Perspectives.Cell biochemistry and biophysics · 2026Review
- Evolutionary innovation within conserved gene regulatory networks underlying biomineralized skeletons in Bilateria.Molecular biology and evolution · 2026Article
- DNA methylation landscape of Nautilus Pompilius.BMC genomics · 2025Article
- Genomic, transcriptomic and epigenomic signatures of ageing and cold adaptation in the Antarctic clamOpen biology · 2025Article
- Annelid methylomes reveal ancestral developmental and aging-associated epigenetic erosion across Bilateria.Genome biology · 2024Article
- Comparative transcriptome analysis reveals molecular damage associated with cryopreservation in Crassostrea angulata D-larvae rather than to cryoprotectant exposure.BMC genomics · 2024Article
- Unravelling epigenetic mechanisms in Cerastoderma edule genome: a comparison of healthy and neoplastic cockles.Molecular genetics and genomics : MGG · 2024Article
- The Effects of Larval Cryopreservation on the Epigenetics of the Pacific OysterInternational journal of molecular sciences · 2023Article
- Functional genomics in Spiralia.Briefings in functional genomics · 2023Article
- mGenomics, proteomics & bioinformatics · 2023Article
- Experimental DNA Demethylation Reduces Expression Plasticity and Thermal Tolerance in Pacific Oysters.Marine biotechnology (New York, N.Y.) · 2023Article
- Epigenetic machinery is functionally conserved in cephalopods.BMC biology · 2022Article
- DNA Methyltransferases: From Evolution to Clinical Applications.International journal of molecular sciences · 2022Review
- Differential DNA methylation in Pacific oyster reproductive tissue in response to ocean acidification.BMC genomics · 2022Article
- Review
- A Broad Survey of Gene Body and Repeat Methylation in Cnidaria Reveals a Complex Evolutionary History.Genome biology and evolution · 2022Article
- Effects of Cadmium Sulfate on the Brown Garden SnailToxics · 2021Article
- Developmental series of gene expression clarifies maternal mRNA provisioning and maternal-to-zygotic transition in a reef-building coral.BMC genomics · 2021Article
- DNA methylation atlas and machinery in the developing and regenerating annelid Platynereis dumerilii.BMC biology · 2021Article
- Inference of DNA methylation patterns in molluscs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation-quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide.
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