ArticleNucleic acids research2013
An efficient strategy for TALEN-mediated genome engineering in Drosophila.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
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- Phosphoproteomics Reveals the AMPK Substrate Network in Response to DNA Damage and Histone Acetylation.Genomics, proteomics & bioinformatics · 2022Article
- Vps13D functions in a Pink1-dependent and Parkin-independent mitophagy pathway.The Journal of cell biology · 2021Article
- Run or Die in the Evolution of New MicroRNAs-Testing the Red Queen Hypothesis on De Novo New Genes.Molecular biology and evolution · 2021Article
- IL‑6 plays a crucial role in epithelial‑mesenchymal transition and pro‑metastasis induced by sorafenib in liver cancer.Oncology reports · 2021Article
- CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering.Genetics research international · 2018Review
- Effect of TALEN-mediated IL-6 knockout on cell proliferation, apoptosis, invasion and anti-cancer therapy in hepatocellular carcinoma (HCC-LM3) cells.Oncotarget · 2017Article
- Generation of TALE nickase-mediated gene-targeted cows expressing human serum albumin in mammary glands.Scientific reports · 2016Article
- Applications of CRISPR-Cas systems in neuroscience.Nature reviews. Neuroscience · 2016Review
- Targeted Mutagenesis of the Hypophysiotropic Gnrh3 in Zebrafish (Danio rerio) Reveals No Effects on Reproductive Performance.PloS one · 2016Article
- Utilization of TALEN and CRISPR/Cas9 technologies for gene targeting and modification.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Genome-editing tools for stem cell biology.Cell death & disease · 2015Review
- Gene targeting by TALEN-induced homologous recombination in goats directs production of β-lactoglobulin-free, high-human lactoferrin milk.Scientific reports · 2015Article
- TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Germline-transmitted genome editing in Arabidopsis thaliana Using TAL-effector-nucleases.PloS one · 2015Article
- Transcription activator-like effector nuclease (TALEN)-mediated female-specific sterility in the silkworm, Bombyx mori.Insect molecular biology · 2014Article
- Site-specific, TALENs-mediated transformation of Bombyx mori.Insect biochemistry and molecular biology · 2014Article
- Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.Genome research · 2014Article
- Targeted genome engineering techniques in Drosophila.Methods (San Diego, Calif.) · 2014Review
- Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In reverse genetics, a gene's function is elucidated through targeted modifications in the coding region or associated DNA cis-regulatory elements. To this purpose, recently developed customizable transcription activator-like effector nucleases (TALENs) have proven an invaluable tool, allowing introduction of double-strand breaks at predetermined sites in the genome. Here we describe a practical and efficient method for the targeted genome engineering in Drosophila. We demonstrate TALEN-mediated targeted gene integration and efficient identification of mutant flies using a traceable marker phenotype. Furthermore, we developed an easy TALEN assembly (easyT) method relying on simultaneous reactions of DNA Bae I digestion and ligation, enabling construction of complete TALENs from a monomer unit library in a single day. Taken together, our strategy with easyT and TALEN-plasmid microinjection simplifies mutant generation and enables isolation of desired mutant fly lines in the F1 generation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.