ArticleInfection and immunity2013
Virulent Shigella flexneri affects secretion, expression, and glycosylation of gel-forming mucins in mucus-producing cells.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Identification of a genomic cluster related to hypersecretion of intestinal mucus and mucinolytic activity of atypical enteropathogenicFrontiers in cellular and infection microbiology · 2024Article
- Article
- Mucin glycans and their degradation by gut microbiota.Glycoconjugate journal · 2023Review
- Molecular mechanisms of Shigella effector proteins: a common pathogen among diarrheic pediatric population.Molecular and cellular pediatrics · 2022Review
- Reciprocal interactions between gut microbiota and autophagy.World journal of gastroenterology · 2021Review
- Effect of Polysaccharides FromFrontiers in pharmacology · 2021Article
- Painong San, a Traditional Chinese Compound Herbal Medicine, Restores Colon Barrier Function on DSS-Induced Colitis in Mice.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Research in a time of enteroids and organoids: how the human gut model has transformed the study of enteric bacterial pathogens.Gut microbes · 2020Review
- Recurrence of primary sclerosing cholangitis after liver transplantation is associated with specific changes in the gut microbiome pretransplant - a pilot study.Transplant international : official journal of the European Society for Organ Transplantation · 2020Article
- Mapping of Shigella flexneri's tissue distribution and type III secretion apparatus activity during infection of the large intestine of guinea pigs.Pathogens and disease · 2019Article
- Gut Microbiota and Colonization Resistance against Bacterial Enteric Infection.Microbiology and molecular biology reviews : MMBR · 2019Review
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model.Infection and immunity · 2019Article
- Food-grade TiOJournal of nanobiotechnology · 2018Article
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Use of Recombinant Mucin Glycoprotein to Assess the Interaction of the Gastric Pathogen Helicobacter pylori with the Secreted Human Mucin MUC5AC.Bioengineering (Basel, Switzerland) · 2017Article
- Structure of a headful DNA-packaging bacterial virus at 2.9 Å resolution by electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Interactions of Intestinal Bacteria with Components of the Intestinal Mucus.Frontiers in cellular and infection microbiology · 2017Review
- Shigella Diversity and Changing Landscape: Insights for the Twenty-First Century.Frontiers in cellular and infection microbiology · 2016Review
- Pathogenic E. coli exploits SslE mucinase activity to translocate through the mucosal barrier and get access to host cells.PloS one · 2015Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Mucin glycoproteins are secreted in large amounts by the intestinal epithelium and constitute an efficient component of innate immune defenses to promote homeostasis and protect against enteric pathogens. In this study, our objective was to investigate how the bacterial enteropathogen Shigella flexneri, which causes bacillary dysentery, copes with the mucin defense barrier. We report that upon in vitro infection of mucin-producing polarized human intestinal epithelial cells, virulent S. flexneri manipulates the secretion of gel-forming mucins. This phenomenon, which is triggered only by virulent strains, results in accumulation of mucins at the cell apical surface, leading to the appearance of a gel-like structure that favors access of bacteria to the cell surface and the subsequent invasion process. We identify MUC5AC, a gel-forming mucin, as a component of this structure. Formation of this gel does not depend on modifications of electrolyte concentrations, induction of trefoil factor expression, endoplasmic reticulum stress, or response to unfolded proteins. In addition, transcriptional and biochemical analyses of infected cells reveal modulations of mucin gene expression and modifications of mucin glycosylation patterns, both of which are induced by virulent bacteria in a type III secretion system-dependent manner. Thus, S. flexneri has developed a dedicated strategy to alter the mucus barrier by targeting key elements of the gel-forming capacity of mucins: gene transcription, protein glycosylation, and secretion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.