ArticleInfection and immunity2013
Surface association of Pht proteins of Streptococcus pneumoniae.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Analysis of novel zinc-binding proteins in the cell wall ofJournal of bacteriology · 2025Article
- The global proteome ofFrontiers in cellular and infection microbiology · 2025Article
- C-Reactive Protein: Pathophysiology, Diagnosis, False Test Results and a Novel Diagnostic Algorithm for Clinicians.Diseases (Basel, Switzerland) · 2023Review
- Immunoinformatics-aided design of a new multi-epitope vaccine adjuvanted with domain 4 of pneumolysin against Streptococcus pneumoniae strains.BMC bioinformatics · 2023Article
- A new candidate epitope-based vaccine against PspA PhtD ofFrontiers in cellular and infection microbiology · 2023Article
- Recent progress in pneumococcal protein vaccines.Frontiers in immunology · 2023Review
- Diverse Mechanisms of Protective Anti-Pneumococcal Antibodies.Frontiers in cellular and infection microbiology · 2022Review
- Broadly Reactive Human Monoclonal Antibodies Targeting the Pneumococcal Histidine Triad Protein Protect against Fatal Pneumococcal Infection.Infection and immunity · 2021Article
- Pneumococcal Vaccines.Microbiology spectrum · 2019Review
- Th17 responses to pneumococcus in blood and adenoidal cells in children.Clinical and experimental immunology · 2019Article
- Article
- Evaluation of Protective Efficacy of Selected Immunodominant B-Cell Epitopes within Virulent Surface Proteins of Streptococcus pneumoniae.Infection and immunity · 2018Article
- Effect of Maternally Derived Anti-protein and Anticapsular IgG Antibodies on the Rate of Acquisition of Nasopharyngeal Carriage of Pneumococcus in Newborns.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- The First Histidine Triad Motif of PhtD Is Critical for Zinc Homeostasis in Streptococcus pneumoniae.Infection and immunity · 2016Article
- The type II histidine triad protein HtpsC is a novel adhesion with the involvement of Streptococcus suis virulence.Virulence · 2015Article
- Human antibodies to PhtD, PcpA, and Ply reduce adherence to human lung epithelial cells and murine nasopharyngeal colonization by Streptococcus pneumoniae.Infection and immunity · 2014Article
- Overlapping functionality of the Pht proteins in zinc homeostasis of Streptococcus pneumoniae.Infection and immunity · 2014Article
- Role of Pht proteins in attachment of Streptococcus pneumoniae to respiratory epithelial cells.Infection and immunity · 2014Article
- Manganese acquisition and homeostasis at the host-pathogen interface.Frontiers in cellular and infection microbiology · 2013Review
- Vaccination against Streptococcus pneumoniae using truncated derivatives of polyhistidine triad protein D.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae is a major human pathogen responsible for massive global morbidity and mortality. The pneumococcus attaches a variety of proteins to its cell surface, many of which contribute to virulence; one such family are the polyhistidine triad (Pht) proteins PhtA, PhtB, PhtD, and PhtE. In this study, we have examined the mechanism of Pht surface attachment using PhtD as a model. Analysis of deletion and point mutants identified a three-amino-acid region of PhtD (Q27-H28-R29) that is critical for the process. The analogous region in PhtE was also necessary for its attachment to the cell surface. Furthermore, we show that a large proportion of the total amount of each Pht protein is released into bacterial culture supernatants. Other surface proteins were also released, albeit to lesser extents, and this was not due to pneumococcal autolysis. The extent of release of surface proteins was strain dependent and was not affected by the capsule. Lastly, we compared the fitness of wild-type and ΔphtABDE pneumococci in vivo in a mouse coinfection model. Release of Pht proteins by the wild type did not complement the mutant strain, consistent with surface-attached rather than soluble forms of the Pht proteins playing the major role in virulence. The significant degree of release of Pht proteins from intact bacteria may have implications for the use of these proteins in novel vaccines.
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