ArticleJournal of molecular biology2013
Low-molecular-weight DNA replication intermediates in Escherichia coli: mechanism of formation and strand specificity.
Article in Journal of molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS.Nucleic acids research · 2024Article
- Genomic landscape of single-stranded DNA gapped intermediates in Escherichia coli.Nucleic acids research · 2022Article
- A Comprehensive View of Translesion Synthesis in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2020Review
- Solution to the 50-year-old Okazaki-fragment problem.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Near-continuously synthesized leading strands inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Replication Fork Breakage and Restart in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2018Review
- When DNA Topology Turns Deadly - RNA Polymerases Dig in Their R-Loops to Stand Their Ground: New Positive and Negative (Super)Twists in the Replication-Transcription Conflict.Trends in genetics : TIG · 2018Review
- Template-switching during replication fork repair in bacteria.DNA repair · 2017Review
- Article
- Bacterial Proliferation: Keep Dividing and Don't Mind the Gap.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Chromosomal DNA replication intermediates, revealed in ligase-deficient conditions in vivo, are of low molecular weight (LMW) independently of the organism, suggesting discontinuous replication of both the leading and the lagging DNA strands. Yet, in vitro experiments with purified enzymes replicating sigma-structured substrates show continuous synthesis of the leading DNA strand in complete absence of ligase, supporting the textbook model of semi-discontinuous DNA replication. The discrepancy between the in vivo and in vitro results is rationalized by proposing that various excision repair events nick continuously synthesized leading strands after synthesis, producing the observed LMW intermediates. Here, we show that, in an Escherichia coli ligase-deficient strain with all known excision repair pathways inactivated, new DNA is still synthesized discontinuously. Furthermore, hybridization to strand-specific targets demonstrates that the LMW replication intermediates come from both the lagging and the leading strands. These results support the model of discontinuous leading strand synthesis in E. coli.
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