ReviewCurrent opinion in chemical biology2013
Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications.
Review in Current opinion in chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed.
- Recent developments of fluorescent proteins and biosensors driven by cross-disciplinary insights.Nature methods · 2026Review
- Non-Covalent Reversibly Photoconvertible Fluorescent Tags for Wash-Free Protein Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A tool to pulse-label yeast nuclear pore complexes in imaging and biochemical experiments.eLife · 2026Article
- Stenhouse Zwitterion Photoswitches as Hydrogen-Bond Acceptors.Journal of the American Chemical Society · 2026Article
- Light-Assisted Akamptisomerization: Excited-State Bond-Angle Reflection (ESBAR) as a Molecular Photoswitching Element in BInorganic chemistry · 2026Article
- A twisted chromophore powers a turn-on fluorescent protein chloride sensor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Optogenetics to biomolecular phase separation in neurodegenerative diseases.Molecules and cells · 2025Review
- Akamptisomerism as a Switching Element: Substituent Effects on Bond Angle Reflection and Photophysical Properties of B-O-B Porphyrins.Inorganic chemistry · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Genetically encoded protein oscillators for FM streaming of single-cell data.bioRxiv : the preprint server for biology · 2025Article
- Article
- Reversible light-responsive protein hydrogel for on-demand cell encapsulation and release.Acta biomaterialia · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Photoactivatable Blue Fluorescent Protein.ACS omega · 2024Article
- Advances in Single-Cell Techniques for Linking Phenotypes to Genotypes.Cancer heterogeneity and plasticity · 2024Article
- Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines.Chemical reviews · 2023Review
- Current Trends of Bacterial and Fungal Optoproteins for Novel Optical Applications.International journal of molecular sciences · 2023Review
- Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.Journal of the American Chemical Society · 2023Article
- Structural Characterization of Fluorescent Proteins Using Tunable Femtosecond Stimulated Raman Spectroscopy.International journal of molecular sciences · 2023Article
- Photophysics of the red-form Kaede chromophore.Chemical science · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Reversibly photoswitchable fluorescent proteins (RSFPs) are fluorescent proteins whose fluorescence, upon excitation at a certain wavelength, can be switched on or off by light in a reversible manner. In the last 10 years, many new RSFPs have been developed and novel applications in cell imaging discovered that rely on their photoswitching properties. This review will describe research on the mechanisms of reversible photoswitching and recent applications using RSFPs. While cis-trans isomerization of the chromophore is believed to be the general mechanism for most RSFPs, structural studies reveal diversity in the details of photoswitching mechanisms, including different effects of protonation, chromophore planarity, and pocket flexibility. Applications of RSFPs include new types of live-cell superresolution imaging, tracking of protein movements and interactions, information storage, and optical control of protein activity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.