ArticleBiochemical and biophysical research communications2013
Biophysical cues enhance myogenesis of human adipose derived stem/stromal cells.
Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02923219 (The Clinical Application of Adipose-Derived Stem Cells on Facial Rejuvenation), which is not on this map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Clinical Application of Adipose-Derived Stem Cells on Facial Rejuvenation
Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Bioprocessing Considerations towards the Manufacturing of Therapeutic Skeletal and Smooth Muscle Cells.Bioengineering (Basel, Switzerland) · 2023Review
- Tissue Engineering Applied to Skeletal Muscle: Strategies and Perspectives.Bioengineering (Basel, Switzerland) · 2022Review
- Adult stem cell sources for skeletal and smooth muscle tissue engineering.Stem cell research & therapy · 2022Review
- Actin polymerization state regulates osteogenic differentiation in human adipose-derived stem cells.Cellular & molecular biology letters · 2021Article
- Protocol for the Use of a Novel Bioreactor System for Hydrated Mechanical Testing, Strained Sterile Culture, and Force of Contraction Measurement of Tissue Engineered Muscle Constructs.Frontiers in cell and developmental biology · 2021Article
- Myogenic Differentiation of Stem Cells for Skeletal Muscle Regeneration.Stem cells international · 2021Review
- Mechanical Stimulation of Adhesion Receptors Using Light-Responsive Nanoparticle Actuators Enhances Myogenesis.ACS applied materials & interfaces · 2020Article
- Recent Advance in Source, Property, Differentiation, and Applications of Infrapatellar Fat Pad Adipose-Derived Stem Cells.Stem cells international · 2020Review
- Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model.Cell transplantation · 2018Article
- Infrapatellar Fat Pad-Derived Stem Cell-Based Regenerative Strategies in Orthopedic Surgery.Knee surgery & related research · 2018Review
- A Poroelastic Model of a Fibrous-Porous Tissue Engineering Scaffold.Scientific reports · 2018Article
- Three-Dimensional Graphene-RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2018Article
- Biophysical Stimulation for Engineering Functional Skeletal Muscle.Tissue engineering. Part B, Reviews · 2017Article
- Modeling Stem Cell Myogenic Differentiation.Scientific reports · 2017Article
- Anisotropic Materials for Skeletal-Muscle-Tissue Engineering.Advanced materials (Deerfield Beach, Fla.) · 2016Review
- Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells.Journal of translational medicine · 2016Article
- Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells.Biomedical engineering online · 2016Review
- Article
- Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes.Biomedical engineering online · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Adipose-derived stem/stromal cell (ASC)-based tissue engineered muscle grafts could provide an effective alternative therapy to autografts - which are limited by their availability - for the regeneration of damaged muscle. However, the current myogenic potential of ASCs is limited by their low differentiation efficiency into myoblasts. The aim of this study was to enhance the myogenic response of human ASCs to biochemical cues by providing biophysical stimuli (11% cyclic uniaxial strain, 0.5 Hz, 1h/day) to mimic the cues present in the native muscle microenvironment. ASCs elongated and fused upon induction with myogenic induction medium alone. Yet, their myogenic characteristics were significantly enhanced with the addition of biophysical stimulation; the nuclei per cell increased approximately 4.5-fold by day 21 in dynamic compared to static conditions (23.3 ± 7.3 vs. 5.2 ± 1.6, respectively), they aligned at almost 45° to the direction of strain, and exhibited significantly higher expression of myogenic proteins (desmin, myoD and myosin heavy chain). These results demonstrate that mimicking the biophysical cues inherent to the native muscle microenvironment in monolayer ASC cultures significantly improves their differentiation along the myogenic lineage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.