ArticleBiomacromolecules2013
ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments.
Article in Biomacromolecules, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A Chemical Framework for Engineering Extracellular Vesicles' Biointerface to Advance Precision Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Strain-Accelerated β-Thiolactone Native Chemical Ligation with Kinetic Control Enables Rapid and Selective Hydrogelation for Biofabrication.Journal of the American Chemical Society · 2026Article
- Ultra-low content physio-chemically crosslinked gelatin hydrogel improves encapsulated 3D cell culture.International journal of biological macromolecules · 2024Article
- A 3D in vitro model of the dermoepidermal junction amenable to mechanical testing.Journal of biomedical materials research. Part A · 2018Article
- Thiol-Mediated Chemoselective Strategies forGels (Basel, Switzerland) · 2018Review
- Engineering Approaches to Study Cellular Decision Making.Annual review of biomedical engineering · 2018Review
- Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments.Stem cells international · 2018Article
- Imaging the Cardiac Extracellular Matrix.Advances in experimental medicine and biology · 2018Review
- Heterogeneous Differentiation of Human Mesenchymal Stem Cells in 3D Extracellular Matrix Composites.BioResearch open access · 2016Article
- An integrated statistical model for enhanced murine cardiomyocyte differentiation via optimized engagement of 3D extracellular matrices.Scientific reports · 2015Article
- Extracellular Matrix-Based Biohybrid Materials for Engineering Compliant, Matrix-Dense Tissues.Advanced healthcare materials · 2015Review
- Grafting Techniques towards Production of Peptide-Tethered Hydrogels, a Novel Class of Materials with Biomedical Interest.Gels (Basel, Switzerland) · 2015Review
- Improved method for synthesis of cysteine modified hyaluronic acid for in situ hydrogel formation.Chemical communications (Cambridge, England) · 2015Article
- In situ vesicle formation by native chemical ligation.Angewandte Chemie (International ed. in English) · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Limiting the precise study of the biochemical impact of whole molecule extracellular matrix (ECM) proteins on stem cell differentiation is the lack of 3D in vitro models that can accommodate many different types of ECM. Here we sought to generate such a system while maintaining consistent mechanical properties and supporting stem cell survival. To this end, we used native chemical ligation to cross-link poly(ethylene glycol) macromonomers under mild conditions while entrapping ECM proteins (termed ECM composites) and stem cells. Sufficiently low concentrations of ECM were used to maintain constant storage moduli and pore size. Viability of stem cells in composites was maintained over multiple weeks. ECM of composites encompassed stem cells and directed the formation of distinct structures dependent on ECM type. Thus, we introduce a powerful approach to study the biochemical impact of multiple ECM proteins (either alone or in combination) on stem cell behavior.
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Registered trials
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