ArticleBiochemistry2013
Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Gravity as a Boundary Condition for the Evolution of Three-Dimensional Multicellularity.Life (Basel, Switzerland) · 2026Article
- Lipid Regulation of Mechanosensitive Ion Channels.International journal of molecular sciences · 2026Review
- Response of heterologously expressed pressure sensor-actuator-modulator macromolecule to external mechanical stress.Heliyon · 2024Article
- Mechanosensitive channel MscL gating transitions coupling with constriction point shift.Protein science : a publication of the Protein Society · 2024Article
- "Force-From-Lipids" Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes.Microorganisms · 2023Article
- Tight hydrophobic core and flexible helices yield MscL with a high tension gating threshold and a membrane area mechanical strain buffer.Frontiers in chemistry · 2023Article
- Cryo-EM Structure of Mechanosensitive Channel YnaI Using SMA2000: Challenges and Opportunities.Membranes · 2021Article
- Mechanical Activation of MscL Revealed by a Locally Distributed Tension Molecular Dynamics Approach.Biophysical journal · 2021Article
- Role of adhesion forces in mechanosensitive channel gating in Staphylococcus aureus adhering to surfaces.NPJ biofilms and microbiomes · 2020Article
- In-Lipid Structure of Pressure-Sensitive Domains Hints Mechanosensitive Channel Functional Diversity.Biophysical journal · 2020Article
- Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.Microbiology and molecular biology reviews : MMBR · 2020Review
- Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets.Nature communications · 2019Article
- Evolutionary specialization of MscCG, an MscS-like mechanosensitive channel, in amino acid transport in Corynebacterium glutamicum.Scientific reports · 2018Article
- The PEG-responding desiccome of the alder microsymbiont Frankia alni.Scientific reports · 2018Article
- Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore.PLoS biology · 2016Article
- Quantifying the stabilizing effects of protein-ligand interactions in the gas phase.Nature communications · 2015Article
- Patch clamp characterization of the effect of cardiolipin on MscS of E. coli.European biophysics journal : EBJ · 2015Article
- Phosphoinositide regulation of TRPV1 revisited.Pflugers Archiv : European journal of physiology · 2015Review
- A new antibiotic with potent activity targets MscL.The Journal of antibiotics · 2015Article
- The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry.Chemistry & biology · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The bacterial mechanosensitive channel of large conductance (MscL) directly senses and responds to membrane tension. It serves as an "emergency release valve" upon acute decreases in the osmotic environment, thus preventing cell lysis. It is one of the best studied mechanosensitive channels and serves as a paradigm of how a channel senses and responds to its membrane environment. The MscL protein is highly conserved, found throughout the bacterial kingdom, and has been shown to encode a functional mechanosensitive channel in all species where it has been studied. However, channels from different species have shown some functional variance; an extreme example is the Mycobacterium tuberculosis MscL, which when heterologously expressed in Escherichia coli requires significantly more membrane tension for gating than the endogenous E. coli MscL. We previously speculated that the membrane environment or factors not found in E. coli promoted the proper gating of the M. tuberculosis MscL channel in its native environment. Here, by reconstituting the M. tuberculosis and E. coli MscL channels in various lipids, we demonstrate that inclusion of phosphatidylinositol, a lipid found in M. tuberculosis but not E. coli, is sufficient for gating of the M. tuberculosis MscL channel within a physiological range of membrane tension.
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