ArticlePloS one2013
Improving precision of proximity ligation assay by amplified single molecule detection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Review detection of Newcastle disease virus.Frontiers in veterinary science · 2022Review
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- Circulating tumour cells and circulating cell-free DNA in patients with lung cancer: a comparison between thoracotomy and video-assisted thoracoscopic surgery.BMJ open respiratory research · 2021Article
- Tumor-Induced Inflammatory Cytokines and the Emerging Diagnostic Devices for Cancer Detection and Prognosis.Frontiers in oncology · 2021Review
- IRX3/5 regulate mitotic chromatid segregation and limb bud shape.Development (Cambridge, England) · 2020Article
- A multiplex platform for digital measurement of circular DNA reaction products.Nucleic acids research · 2020Article
- Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays.Angewandte Chemie (International ed. in English) · 2020Review
- Fingerprinting Non-Terran Biosignatures.Astrobiology · 2018Article
- Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- AKAP12 mediates PKA-induced phosphorylation of ATR to enhance nucleotide excision repair.Nucleic acids research · 2016Article
- KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection.PLoS pathogens · 2016Article
- Proximity assays for sensitive quantification of proteins.Biomolecular detection and quantification · 2015Review
- A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors.The Journal of biological chemistry · 2015Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.