ArticlePloS one2013
The intracellular citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' encodes two novel autotransporters.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 36 citations in OpenAlex.
- Molecular Characterization of 'Microorganisms · 2025Article
- Time-Course Gene Expression of 'Microorganisms · 2025Article
- Differential expression of "Candidatus Liberibacter solanacearum" genes and prophage loci in different life stages of potato psyllid.Scientific reports · 2024Article
- Pathogenicity and Transcriptomic Analyses of Two "Microbiology spectrum · 2023Article
- Interactions ofInternational journal of molecular sciences · 2022Review
- Two Liberibacter Proteins Combine to Suppress Critical Innate Immune Defenses in Citrus.Frontiers in plant science · 2022Article
- The Mechanism of Citrus Host Defense Response Repression at Early Stages of Infection by Feeding ofFrontiers in plant science · 2021Article
- Zinkicide Is a ZnO-Based Nanoformulation with Bactericidal Activity against Liberibacter crescens in Batch Cultures and in Microfluidic Chambers Simulating Plant Vascular Systems.Applied and environmental microbiology · 2020Article
- Review
- The Ferredoxin-Like Protein FerR Regulates PrbP Activity inApplied and environmental microbiology · 2019Article
- Nuclear Import of a Secreted "Frontiers in microbiology · 2019Article
- The enemy within: phloem-limited pathogens.Molecular plant pathology · 2018Review
- PAMPs, PRRs, effectors and R-genes associated with citrus-pathogen interactions.Annals of botany · 2017Review
- Molecular mechanisms behind the accumulation of ATP and HHorticulture research · 2017Article
- Vector-Borne Bacterial Plant Pathogens: Interactions with Hemipteran Insects and Plants.Frontiers in plant science · 2016Review
- Transient Expression of Candidatus Liberibacter Asiaticus Effector Induces Cell Death in Nicotiana benthamiana.Frontiers in plant science · 2016Article
- Extracellular protease digestion to evaluate membrane protein cell surface localization.Nature protocols · 2015Article
- Genetic diversity of Candidatus Liberibacter asiaticus based on two hypervariable effector genes in Thailand.PloS one · 2014Article
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4 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Proteins secreted by the type V secretion system (T5SS), known as autotransporters, are large extracellular virulence proteins localized to the bacterial poles. In this study, we characterized two novel autotransporter proteins of 'Candidatus Liberibacter asiaticus' (Las), and redesignated them as LasAI and LasAII in lieu of the previous names HyvI and HyvII. As a phloem-limited, intracellular bacterial pathogen, Las has a significantly reduced genome and causes huanglongbing (HLB), a devastating disease of citrus worldwide. Bioinformatic analyses revealed that LasAI and LasAII share the structural features of an autotransporter family containing large repeats of a passenger domain and a unique C-terminal translocator domain. When fused to the GFP gene and expressed in E. coli, the LasAI C-terminus and the full length LasAII were localized to the bacterial poles, similar to other members of autotransporter family. Despite the absence of a typical signal peptide, LasAI was found to localize at the cell surface by immuno-dot blot using a monoclonal antibody against the partial LasAI protein. Its surface localization was also confirmed by the removal of the LasAI antigen using a proteinase K treatment of the intact bacterial cells. When co-inoculated with a P19 gene silencing suppressor and transiently expressed in tobacco leaves, the GFP-LasAI translocator targeted to the mitochondria. This is the first report that Las encodes novel autotransporters that target to mitochondria when expressed in the plants. These findings may lead to a better understanding of the pathogenesis of this intracellular bacterium.
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