ArticlePloS one2013
Systems biology analysis of Zymomonas mobilis ZM4 ethanol stress responses.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering.Microbial cell factories · 2024Review
- Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis.Biotechnology for biofuels and bioproducts · 2024Article
- Molecular mechanism of enhanced ethanol tolerance associated withFrontiers in bioengineering and biotechnology · 2022Article
- A plasmid-freeFrontiers in bioengineering and biotechnology · 2022Article
- Genome-Scale Transcription-Translation Mapping Reveals Features of Zymomonas mobilis Transcription Units and Promoters.mSystems · 2020Article
- Physiological effects of overexpressed sigma factors on fermentative stress response of Zymomonas mobilis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Multiple Small RNAs Interact to Co-regulate Ethanol Tolerance inFrontiers in bioengineering and biotechnology · 2020Article
- Impact of hfq and sigE on the tolerance of Zymomonas mobilis ZM4 to furfural and acetic acid stresses.PloS one · 2020Article
- Metabolic engineering ofBiotechnology for biofuels · 2020Article
- Transcriptomic Profiles ofFrontiers in microbiology · 2020Article
- Bioethanol production from microalgae polysaccharides.Folia microbiologica · 2019Review
- Phenotypic and genomic analysis ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Prediction and characterization of promoters and ribosomal binding sites ofBiotechnology for biofuels · 2019Article
- Characterization of a Dimeric Arginase FromFrontiers in microbiology · 2019Article
- Applying a New REFINE Approach inFrontiers in microbiology · 2019Article
- Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance inBiotechnology for biofuels · 2018Article
- Complete genome sequence and the expression pattern of plasmids of the model ethanologenBiotechnology for biofuels · 2018Article
- Identification and Characterization of 5' Untranslated Regions (5'UTRs) inFrontiers in microbiology · 2017Article
- Zymomonas mobilis as a model system for production of biofuels and biochemicals.Microbial biotechnology · 2016Review
- Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis.Microbial cell factories · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundZymomonas mobilis ZM4 is a capable ethanologenic bacterium with high ethanol productivity and ethanol tolerance. Previous studies indicated that several stress-related proteins and changes in the ZM4 membrane lipid composition may contribute to ethanol tolerance. However, the molecular mechanisms of its ethanol stress response have not been elucidated fully. METHODOLOGY/PRINCIPAL
findingsIn this study, ethanol stress responses were investigated using systems biology approaches. Medium supplementation with an initial 47 g/L (6% v/v) ethanol reduced Z. mobilis ZM4 glucose consumption, growth rate and ethanol productivity compared to that of untreated controls. A proteomic analysis of early exponential growth identified about one thousand proteins, or approximately 55% of the predicted ZM4 proteome. Proteins related to metabolism and stress response such as chaperones and key regulators were more abundant in the early ethanol stress condition. Transcriptomic studies indicated that the response of ZM4 to ethanol is dynamic, complex and involves many genes from all the different functional categories. Most down-regulated genes were related to translation and ribosome biogenesis, while the ethanol-upregulated genes were mostly related to cellular processes and metabolism. Transcriptomic data were used to update Z. mobilis ZM4 operon models. Furthermore, correlations among the transcriptomic, proteomic and metabolic data were examined. Among significantly expressed genes or proteins, we observe higher correlation coefficients when fold-change values are higher.
conclusionsOur study has provided insights into the responses of Z. mobilis to ethanol stress through an integrated "omics" approach for the first time. This systems biology study elucidated key Z. mobilis ZM4 metabolites, genes and proteins that form the foundation of its distinctive physiology and its multifaceted response to ethanol stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.