ArticlePloS one2013
Super resolution microscopy reveals that caveolin-1 is required for spatial organization of CRFB1 and subsequent antiviral signaling in zebrafish.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- A deep learning-based tool for the automated detection and analysis of caveolae in transmission electron microscopy images.Computational and structural biotechnology journal · 2023Article
- The Development and Application of Opto-Chemical Tools in the Zebrafish.Molecules (Basel, Switzerland) · 2022Review
- Super-resolution analysis of PACSIN2 and EHD2 at caveolae.PloS one · 2022Article
- Modeling Virus-Induced Inflammation in Zebrafish: A Balance Between Infection Control and Excessive Inflammation.Frontiers in immunology · 2021Review
- Caveolin elastin-like polypeptide fusions mediate temperature-dependent assembly of caveolar microdomains.ACS biomaterials science & engineering · 2020Article
- Interferon Receptor Trafficking and Signaling: Journey to the Cross Roads.Frontiers in immunology · 2020Review
- Caveolae as Potential Hijackable Gates in Cell Communication.Frontiers in cell and developmental biology · 2020Review
- Caveolae and scaffold detection from single molecule localization microscopy data using deep learning.PloS one · 2019Article
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly.Journal of cell science · 2017Article
- Super-resolution Visualization of Caveola Deformation in Response to Osmotic Stress.The Journal of biological chemistry · 2017Article
- Roles of the Mevalonate Pathway and Cholesterol Trafficking in Pulmonary Host Defense.Current molecular pharmacology · 2017Article
- Single epicardial cell transcriptome sequencing identifies Caveolin 1 as an essential factor in zebrafish heart regeneration.Development (Cambridge, England) · 2016Article
- A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance.Developmental and comparative immunology · 2015Article
- Downregulation of caveolin-1 in chronic rhinosinusitis with and without nasal polyps.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
- Nanoscale imaging of caveolin-1 membrane domains in vivo.PloS one · 2015Article
- Dances with Membranes: Breakthroughs from Super-resolution Imaging.Current topics in membranes · 2015Review
- Influenza A virus infection in zebrafish recapitulates mammalian infection and sensitivity to anti-influenza drug treatment.Disease models & mechanisms · 2014Article
- Membrane distribution of the glycine receptor α3 studied by optical super-resolution microscopy.Histochemistry and cell biology · 2014Article
- In vivo cell biology in zebrafish - providing insights into vertebrate development and disease.Journal of cell science · 2014Review
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Understanding spatial distribution and dynamics of receptors within unperturbed membranes is essential for elucidating their role in antiviral signaling, but conventional studies of detergent-resistant membrane fractions cannot provide this information. Caveolae are integral to numerous signaling pathways and these membrane domains have been previously implicated in viral entry but not antiviral defense. This study shows, for the first time, the importance of spatio-temporal regulation of signaling receptors and the importance of the regulation of clustering for downstream signaling. A novel mechanism for virus evasion of host cell defenses is demonstrated through disruption of clusters of signaling molecules organized within caveolin-rich domains. Viral infection leads to a downregulation in Caveolin-1b (Cav-1b), disrupting clusters of CRFB1, a zebrafish type I interferon receptor (-R) subunit. Super-resolution microscopy has enabled the first single-molecule imaging of CRFB1 association with cav-1b-containing membrane domains. Strikingly, downregulation of Cav-1b, the major protein component of caveolae, caused CRFB1 clusters to disperse. Dispersal of CRFB1 clusters led to a suppressed antiviral immune response both in vitro and in vivo, through abrogation of downstream signaling. This response strongly suggests that CRFB1 organization within cav-1b-containing membrane domains is critical for IFN-mediated antiviral defense and presents a previously undescribed antiviral evasion strategy to alter IFN signaling and the antiviral immune response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.