ArticlePloS one2013
Hepatitis B virus s protein enhances sperm apoptosis and reduces sperm fertilizing capacity in vitro.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- From infection to infertility: a review of the role of human papillomavirus-induced oxidative stress on reproductive health and infertility.European journal of medical research · 2025Review
- The effects of oxidative stress and intracellular calcium on mitochondrial permeability transition pore formation in equine spermatozoa.FASEB bioAdvances · 2024Article
- Update on known and emergent viruses affecting human male genital tract and fertility.Basic and clinical andrology · 2024Review
- Human seminal virome: a panel based on recent literature.Basic and clinical andrology · 2022Review
- Viral Infections and Male Infertility: A Comprehensive Review of the Role of Oxidative Stress.Frontiers in reproductive health · 2022Review
- The Microbiome, an Important Factor That Is Easily Overlooked in Male Infertility.Frontiers in microbiology · 2022Review
- Viral tropism for the testis and sexual transmission.Frontiers in immunology · 2022Review
- Article
- Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo.Reproductive toxicology (Elmsford, N.Y.) · 2017Article
- Oxidative damage of DNA induced by X-irradiation decreases the uterine endometrial receptivity which involves mitochondrial and lysosomal dysfunction.International journal of clinical and experimental medicine · 2015Article
- Viral infections and implications for male reproductive health.Asian journal of andrologyArticle
- Transcription and regulation of hepatitis B virus genes in host sperm cells.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveStudying the impact of Hepatitis B virus S protein (HBs) on early apoptotic events in human spermatozoa and sperm fertilizing capacity. METHODOLOGY/PRINCIPAL
findingsSpermatozoa were exposed to HBs (0, 25, 50, 100 µg/ml) for 3 h, and then fluo-4 AM calcium assay, Calcein/Co(2+) assay, protein extraction and ELISA, ADP/ATP ratio assay, sperm motility and hyperactivation and sperm-zona pellucida (ZP) binding and ZP-induced acrosome reaction (ZPIAR) tests were performed. The results showed that in the spermatozoa, with increasing concentration of HBs, (1) average cytosolic free Ca(2+) concentration ([Ca(2+)]i) rose; (2) fluorescence intensity of Cal-AM declined; (3) average levels of cytochrome c decreased in mitochondrial fraction and increased in cytosolic fraction; (4) ADP/ATP ratios rose; (5) average rates of total motility and mean hyperactivation declined; (6) average rate of ZPIAR declined. In the above groups the effects of HBs exhibited dose dependency. However, there was no significant difference in the number of sperms bound to ZP between the control and all test groups.
conclusionHBs could induce early events in the apoptotic cascade in human spermatozoa, such as elevation of [Ca(2+)]i, opening of mitochondrial permeability transition pore (MPTP), release of cytochrome c (cyt c) and increase of ADP/ATP ratio, but exerted a negative impact on sperm fertilizing capacity.
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Registered trials
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