ArticlePloS one2013
Evaluation of reference genes for RT-qPCR expression studies in hop (Humulus lupulus L.) during infection with vascular pathogen verticillium albo-atrum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Genome-wide DNA methylation analysis of CBCVd-infected hop plants (Microbiology spectrum · 2025Article
- Current status and future perspectives of the diagnostic of plant bacterial pathogens.Frontiers in plant science · 2025Review
- The hops (Humulus lupulus) genome contains a mid-sized terpene synthase family that shows wide functional and allelic diversity.BMC plant biology · 2023Article
- Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging withInternational journal of molecular sciences · 2021Article
- Changes in the Phenolic Compounds of Hop (Plants (Basel, Switzerland) · 2020Article
- Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants.International journal of molecular sciences · 2020Article
- Expression of Putative Defense Responses in Cannabis Primed byFrontiers in plant science · 2020Article
- Evaluation of Disease Severity and Global Transcriptome Response Induced byInternational journal of molecular sciences · 2019Article
- Revisiting the Role of Transcription Factors in Coordinating the Defense Response AgainstViruses · 2019Article
- Virus-induced gene silencing (VIGS) inPlant methods · 2019Article
- Validation of reference genes for RT-qPCR studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes.BMC genomics · 2018Article
- Comparative transcriptional analysis of hop responses to infection with Verticillium nonalfalfae.Plant cell reports · 2017Article
- Article
- Article
- Identification and Validation of Reference Genes for RT-qPCR Analysis in Non-Heading Chinese Cabbage Flowers.Frontiers in plant science · 2016Article
- Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Lycoris aurea.Frontiers in plant science · 2016Article
- Article
- Isolation of high quality RNA from pistachio (Pistacia vera L.) and other woody plants high in secondary metabolites.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Genome-Wide Identification and Validation of Reference Genes in Infected Tomato Leaves for Quantitative RT-PCR Analyses.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hop plant (Humulus lupulus L.), cultivated primarily for its use in the brewing industry, is faced with a variety of diseases, including severe vascular diseases, such as Verticillium wilt, against which no effective protection is available. The understanding of disease resistance with tools such as differentially expressed gene studies is an important objective of plant defense mechanisms. In this study, we evaluated twenty-three reference genes for RT-qPCR expression studies on hop under biotic stress conditions. The candidate genes were validated on susceptible and resistant hop cultivars sampled at three different time points after infection with Verticillium albo-atrum. The stability of expression and the number of genes required for accurate normalization were assessed by three different Excel-based approaches (geNorm v.3.5 software, NormFinder, and RefFinder). High consistency was found among them, identifying the same six best reference genes (YLS8, DRH1, TIP41, CAC, POAC and SAND) and five least stably expressed genes (CYCL, UBQ11, POACT, GAPDH and NADH). The candidate genes in different experimental subsets/conditions resulted in different rankings. A combination of the two best reference genes, YLS8 and DRH1, was used for normalization of RT-qPCR data of the gene of interest (PR-1) implicated in biotic stress of hop. We outlined the differences between normalized and non-normalized values and the importance of RT-qPCR data normalization. The high correlation obtained among data standardized with different sets of reference genes confirms the suitability of the reference genes selected for normalization. Lower correlations between normalized and non-normalized data may reflect different quantity and/or quality of RNA samples used in RT-qPCR analyses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.