ArticlePloS one2013
Identification and characterization of microRNAs from Entamoeba histolytica HM1-IMSS.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Entamoeba histolytica extracellular vesicles drive pro-inflammatory monocyte signaling.PLoS neglected tropical diseases · 2025Article
- miRNA, New Perspective to World of Intestinal Protozoa and Toxoplasma.Acta parasitologica · 2024Review
- microRNAs: Critical Players during Helminth Infections.Microorganisms · 2022Review
- In Silico Identification and Characterization of circRNAs as Potential Virulence-Related miRNA/siRNA Sponges fromNon-coding RNA · 2022Article
- RNA Sequencing Reveals Widespread Transcription of Natural Antisense RNAs inMicroorganisms · 2022Article
- Parasite-derived microRNAs as a diagnostic biomarker: potential roles, characteristics, and limitations.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Review
- Human microRNAs in host-parasite interaction: a review.3 Biotech · 2020Review
- Genomic determinants for initiation and length of natural antisense transcripts in Entamoeba histolytica.Scientific reports · 2020Article
- DetectingJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Genetic Diversity and Gene Family Expansions in Members of the Genus Entamoeba.Genome biology and evolution · 2019Article
- Postsplicing-Derived Full-Length Intron Circles in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2018Article
- Serum stress responsive gene EhslncRNA of Entamoeba histolytica is a novel long noncoding RNA.Scientific reports · 2016Article
- Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.Genome research · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEntamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation. METHODOLOGY/PRINCIPAL
findingsWe sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequence reads. Based on a bioinformatics pipeline, we found that only 0.5% of these non-redundant small RNA reads were a perfect match with the drafted E. histolytica genome. We did not find miRNA homologs in plant or animal miRNAs. We discovered 199 new potential Entamoeba histolytica miRNAs. The expression and sequence of these Ehi-miRNAs were further validated through microarray by µParaflo Microfluidic Biochip Technology. Ten potential miRNAs were additionally confirmed by real time RT-PCR analysis. Prediction of target genes matched 32 known genes and 34 hypothetical genes. CONCLUSIONS/SIGNIFICANCE: These results show that there is a number of regulatory miRNAs in Entamoeba histolytica. The collection of miRNAs in this parasite could be used as a new platform to study genomic structure, gene regulation and networks, development, and host-parasite interactions.
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