ArticleFrontiers in microbiology2013
Molecular characterization of bla ESBL-harboring conjugative plasmids identified in multi-drug resistant Escherichia coli isolated from food-producing animals and healthy humans.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 2 of them syntheses that pooled it.
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Who cites it
48 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pooled it
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- Genomic characterization of multidrug-resistant Escherichia coli strains identified from patients with urinary tract infection in Egypt.Scientific reports · 2026Article
- Characterization of extended-spectrum cephalosporin-resistant Klebsiella recovered from dairy manure in Southern Ontario, Canada.PloS one · 2026Article
- Evolutionary trends and genetic determinants of third-generation cephalosporin resistance inFrontiers in microbiology · 2026Article
- Occurrence and Molecular Characterization of Extended-Spectrum Beta-Lactamase (ESBL)-ProducingAntibiotics (Basel, Switzerland) · 2025Article
- High prevalence ofApplied and environmental microbiology · 2025Article
- Interplay between bacterial clones and plasmids in the spread ofOne health (Amsterdam, Netherlands) · 2025Article
- Prevalence of ESBL-Resistant Genes in Birds in Italy-A Comprehensive Review.Animals : an open access journal from MDPI · 2025Review
- Prevalence and genetic basis of extended-spectrum β-lactamase-producingFrontiers in veterinary science · 2025Article
- Antimicrobial Resistance Profile ofActa medica Philippina · 2025Article
- Comprehensive epidemiological profiling of poultry-derivedFrontiers in microbiology · 2025Article
- Exogenous plasmid capture to characterize tetracycline-resistance plasmids in sprouts obtained from retail in Germany.Frontiers in microbiology · 2025Article
- Co-localization of antibiotic resistance genes is widespread in the infant gut microbiome and associates with an immature gut microbial composition.Microbiome · 2024Article
- Article
- Whole genome-based genetic insights ofMicrobiology spectrum · 2023Article
- Extended-Spectrum β-Lactamases (ESBL) Producing Bacteria in Animals.Antibiotics (Basel, Switzerland) · 2023Review
- Evolution of ß-lactams, fluroquinolones and colistin resistance and genetic profiles inItalian journal of food safety · 2022Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundExtended-spectrum β-lactamase (ESBL)-encoding genes are frequently mapped to plasmids, yet few of these structures have been characterized at the molecular level, to date.
methodsEighty-seven ESBL-producing Escherichia coli were isolated from fecal samples of food-producing animals and healthy humans in Switzerland from 2009 to 2011. Plasmid DNA of all isolates was purified. Broth mating assays were carried out individually for 32 isolates to determine if the ESBL marker could be transferred by conjugation. The plasmid sizes were determined by S1-nuclease pulsed-field gel electrophoresis (PFGE) and the plasmids were typed by PCR-based replicon typing. Susceptibility tests by disk diffusion followed with a re-analysis S1-nuclease PFGE and PCRs were performed to confirm plasmid transfer. Microarray was performed to detect additional antibiotic resistance markers and multi-locus sequence typing was also performed in selected donor strains. The phylotypes were identified by triplex PCR.
resultsAbout half (n = 46) of the 87 isolates carried small (<20-kb) plasmids. All selected 32 isolates contained large plasmids (ranging in sizes from 20- to 600-kb). Eleven plasmid replicon types were detected. Of these, IncFIA (n = 5), IncFIB (n = 9), and IncK/B (n = 4) were common. Nine isolates demonstrated the ability to transfer their cefotaxime resistance marker at high transfer rates. Plasmid profile re-analysis of these transconjugants identified 16 plasmids. IncFIB and IncI1 were the most prevalent replicon types. Phylogenetic grouping showed that five of the nine donor strains belonged to phylogroup B1. Nine different sequence types were identified in nine tested donor strains.
conclusionCharacterization of these ESBL-encoding conjugative plasmids extends our understanding on these resistance markers in multi-drug resistant E. coli cultured from healthy human and animal sources.
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